Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.

Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S
Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
46965	pK7WGF2::hCas9	Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
46966	pICH86966::AtU6p::sgRNA_PDS	Expresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter
46968	pICSL01009::AtU6p	Encodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.