A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts.
Bose AK, Janes KA
Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 47573 | pGEX-4T-1 3xFlag-ERK2 | Bacterial expression plasmid for GST-3xFlag-tagged ERK2 |
| 47574 | pGEX-4T-1 3xFlag-JNK1a1 | Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1 |
| 47575 | pGEX-4T-1 3xFlag-p38alpha | Bacterial expression plasmid for GST-3xFlag-tagged p38alpha |
| 47576 | pGEX-4T-1 3xHA-MEK1-DD | Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1 |
| 47577 | pGEX-4T-1 3xHA-MKK4-EE | Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4 |
| 47578 | pGEX-4T-1 3xHA-MKK7a1-EE | Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1 |
| 47579 | pGEX-4T-1 3xHA-MKK6-EE | Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6 |
| 47580 | pCDFDuet-1 MKK4(EE)-MKK7a1(EE) | Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1 |