A high-throughput assay for phosphoprotein-specific phosphatase activity in cellular extracts.

Bose AK, Janes KA
Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 10.1074/mcp.O112.024059. Epub 2012 Dec 11. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
47580	pCDFDuet-1 MKK4(EE)-MKK7a1(EE)	Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1
47579	pGEX-4T-1 3xHA-MKK6-EE	Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK6
47578	pGEX-4T-1 3xHA-MKK7a1-EE	Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1
47577	pGEX-4T-1 3xHA-MKK4-EE	Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK4
47576	pGEX-4T-1 3xHA-MEK1-DD	Bacterial expression plasmid for GST-3xHA-tagged constitutively active MEK1
47575	pGEX-4T-1 3xFlag-p38alpha	Bacterial expression plasmid for GST-3xFlag-tagged p38alpha
47574	pGEX-4T-1 3xFlag-JNK1a1	Bacterial expression plasmid for GST-3xFlag-tagged JNK1a1
47573	pGEX-4T-1 3xFlag-ERK2	Bacterial expression plasmid for GST-3xFlag-tagged ERK2