Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system.
Bikard D, Jiang W, Samai P, Hochschild A, Zhang F, Marraffini LA
Nucleic Acids Res. 2013 Jun 12.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 46569 | pdCas9 | Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers. |
| 46570 | pWJ66 | Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9) |
| 46571 | pWJ68 | Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9) |