RNA-Guided Human Genome Engineering via Cas9.

Mali P, Yang L, Esvelt KM, Aach J, Guell M, Dicarlo JE, Norville JE, Church GM
Science. 2013 Feb 15;339(6121):823-6. doi: 10.1126/science.1232033 PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
41815	hCas9	Expresses human codon optimized Cas9 nuclease for genome engineering
41816	hCas9_D10A	Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
41817	gRNA_AAVS1-T1	Expresses a guide RNA (gRNA) to target human AAVS1 (T1 target sequence) for genome engineering
41818	gRNA_AAVS1-T2	Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering
41819	gRNA_GFP-T1	Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering
41820	gRNA_GFP-T2	Expresses a guide RNA (gRNA) to target GFP (T2 target sequence) for genome engineering
41821	gRNA_DNMT3a-T1	Expresses a guide RNA (gRNA) to target DNMT3a (T1 target sequence) for genome engineering
41822	gRNA_DNMT3a-T2	Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering
41823	gRNA_DNMT3b	Expresses a guide RNA (gRNA) to target DNMT3b for genome engineering
41824	gRNA_Cloning Vector	An empty gRNA expression vector, used to create a gRNA to a specific sequence.