Generation of stable Drosophila cell lines using multicistronic vectors.
Gonzalez M, Martin-Ruiz I, Jimenez S, Pirone L, Barrio R, Sutherland JD
Sci Rep. 2011;1:75. doi: 10.1038/srep00075. Epub 2011 Aug 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 32425 | Ac5-STABLE1-neo | |
| 32426 | Ac5-STABLE2-neo | |
| 208031 | Ac5-STABLE1-puro | Enables expression of an ORF (with or without tags) and selection with puromycin in insect cells. Includes GFP-T2A-puro with Actin5C promoter. |
| 208032 | Ac5-STABLE2-puro | Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter. |
| 208033 | Ac5-STABLE1-blast | Enables expression of an ORF (with or without tags) and selection with blasticidin in insect cells. Includes GFP-T2A-blast with Actin5C promoter. |
| 208034 | Ac5-STABLE2-blast | Enables expression of 2 ORFs (with or without tags) and selection with blasticidin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-blast with Actin5C promoter. |