A multicassette Gateway vector set for high throughput and comparative analyses in ciona and vertebrate embryos.

Roure A, Rothbacher U, Robin F, Kalmar E, Ferone G, Lamy C, Missero C, Mueller F, Lemaire P
PLoS One. 2007. 2(9):e916. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
186350	pDONR-221-P3-P5	Gateway donor vector with AttP3 AttP5 recombination sites
186351	pDONR-221-P3-P4	Gateway donor vector with AttP3 AttP4 recombination sites
186355	pENT-L1-Kozak-EGFP-GPI-stop-L2	Entry clone with ORF encoding plasma membrane-targeted EGFP-GPI fusion protein flanked by Gateway recombination sequences
186356	pENT-L1-Kozak-GAP43-ECFP-stop-L2	Entry clone with ORF encoding plasma membrane-targeted GAP43-eCFP flanked by Gateway recombination sequences
186357	pENT-L1-Kozak-HistoneH2B-L2	Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
186358	pENT-L1-Kozak-NLSlacZ-stop-L2	Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.
186359	pENT-L1-Kozak-lacZ-stop-L2	Entry clone with ORF encoding lacZ flanked by Gateway recombination sequences.
186360	pENT-L1-Kozak-Venus-stop-L2	Entry clone with ORF encoding Venus fluorescent protein flanked by Gateway recombination sequences
186361	pENT-L1-Kozak-Ensconsin-3XGFP-stop-L2	Entry clone with ORF encoding the MT-binding domain of Ensconsin fused to 3 copies of GFP in C-terminal, flanked by Gateway recombination sequences.
186362	pENT-L1-Kozak-Ark-L2	Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.
186364	pENT-L3-pFOGl-L5	Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences
186365	pENT-L3-pSnail-L5	Entry clone with ORF encoding Ci-Snail cis-regulatory region flanked by Gateway recombination sequences
186366	pENT-L3-a-element-L4	Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences
186367	pENT-L3-Titf-L4	Entry clone with ORF encoding Titf1 cis-regulatory region flanked by Gateway recombination sequences
186368	pENT-L3-eBra-L4	Entry clone with ORF encoding Brachury cis-regulatory region flanked by Gateway recombination sequences
186369	pENT-R4-bpFOG-L5	Entry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequences
186370	pDEST-Spe3-RfA	Destination vector for the expression of a protein of interest into embryos, by mRNA microinjection
186371	pDEST-Spe3-Venus-RfA	Destination vector for the expression of a fluorescent fusion of N-ter Venus and a protein of interest into embryos, by mRNA microinjection
186372	pDEST-Spe3-RfA-Venus	Destination vector for the expression of a fusion of an N-ter protein of interest with Venus into embryos, by mRNA microinjection
186373	pDEST-Spe3-HA-RfA	Destination vector for the expression of a fusion of N-ter HA tag and a protein of interest into embryos, by mRNA microinjection
186374	pDEST-Spe3-RfA-HA	Destination vector for the expression of a fusion of an N-ter protein of interest with an HA tag into embryos, by mRNA microinjection
186396	pDEST-pSP172BSSPE-Swa1::RfA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of a regulatory sequence cloned in the Swa1 restriction site.
186397	pDEST-pSP172BSSPE-pFOGc::RfA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned gene of interest under control of the pFOG ectodermal regulatory sequence.
186398	pDEST-pSP172BSSPE-Swa1::venus-RfA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
186400	pDEST-pSP172BSSPE-pFOGc::venus-RfA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.
186401	pDEST-pSP172BSSPE-pFOGc::RfA-venus	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.
186402	pDEST-pSP172BSSPE-Swa1::RfA-ECFP	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.
186403	PDEST-pSP172BSSPE-pFOGc::RfA-ECFP	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.
186404	pDEST-pSP172BSSPE-Swa1::HA-RfA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
186405	pDEST-pSP172BSSPE-Swa1::RfA-HA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.
186406	PDEST-pSP172BSSPE-pFOGc::RfA-HA	Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.
186407	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.
186408	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.
186409	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-venus	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter Venus-gene of interest under control of an AttB3/B5 recombined reg. sequence.
186410	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-HA	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter HA tag under control of an AttB3/B5 recombined reg. sequence.
186411	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.
186412	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.
186413	pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2	Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.
186414	pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2	AttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.