Spatiotemporal control of cell signalling using a light-switchable protein interaction.
Levskaya A, Weiner OD, Lim WA, Voigt CA
Nature. 2009 Oct 15;461(7266):997-1001.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 22275 | pAL149 | Expresses PhyB-mCherry with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane |
| 22276 | pAL175 | Expresses YFP-PIF for red-light-induced binding to Phy |
| 22277 | pAL188 | Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment |
| 22278 | pAL189 | Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment |
| 22279 | pAL190 | Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment |
| 22280 | pAL197 | |
| 22281 | pAL198 | |
| 22282 | pAL204 | Expresses PhyB-mCFP with prenylation sequence from Kras, for red-light-induced recruitment of PIF to the plasma membrane |
| 22471 | pAL113 | Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6. |
| 22474 | pAL101 | Expresses APB domain of PIF6 fused to mYFP, for red-light-induced binding to Phy |
| 22475 | pAL130 | Expresses N terminal domain of PIF3 fused to mYFP, for red-light-induced binding to Phy |