Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists
Hollingsworth LR, Veeraraghavan P, Paulo JA, Harper JW
bioRxiv 2024.04.19.590338
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 214983 | pLV UBC APEX2-3xFLAG-IRES-eGFP | Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments). |
| 214989 | mNeonGreen-P2A-3xFLAG-hEEA1 HDR template | Endogenously tag human EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting. |
| 214990 | mNeonGreen-P2A-3xFLAG-msEEA1 HDR template | Endogenously tag mouse EEA1 with an N-terminal 3xFLAG in addition to cytosolic mNG for cell sorting. |
| 214991 | pTwist-msEEA1 | Gateway entry vector with mouse EEA1 |
| 214994 | pLV-UBC mScarlet-I-P2A-3xFLAG-hEEA1 | Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mScarlet-I to sort for even expression levels. |
| 214995 | pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1 | Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels. |
| 214996 | pLV UBC hTMEM115-3C-3xHA-P2A-eGFP | Stably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels. |
| 214997 | pLV UBC msTMEM115-3C-3xHA-P2A-eGFP | Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels. |
| 214998 | hTMEM115-TEV-3xHA-P2A-mScarlet HDR template | Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting. |
| 214999 | hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template | Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting. |
| 215000 | pTwist Amp msTMEM115-TEV-3C-P2A-mScarlet-I HDR Template | Endogenously tag mouse TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting. |
| 218633 | pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP | Proximity biotinylation of msNLRP3 |
| 218634 | pLV UBC msNLRP3(ΔPYD, I125M-END)-APEX2-3xFLAG-IRES-eGFP | Proximity biotinylation of msNLRP3(ΔPYD, I125M-END) |
| 218635 | pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E) | Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E) |
| 218636 | pLV UBC msNLRP3(LRRm5)-APEX2-3xFLAG-IRES-eGFP (H781E_Q782R_F785A) | Proximity biotinylation of msNLRP3(LRRm5, H781E_Q782R_F785A) |
| 218637 | pLV UBC OSBP(PH)-APEX2-3xFLAG-IRES-eGFP | Proximity biotinylation control of OSBP(PH domain) |
| 218638 | pDONR221 msNLRP3 (STOP) | Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional) |
| 218639 | pDONR221 msNLRP3 | Gateway shuttling vector for cloning C-terminal NLRP3 tags (contains no stop codon). |
| 218640 | pDONR221 msNLRP3(ΔPYD, I125M-END) (STOP) | Gateway shuttling vector for cloning N-terminal NLRP3(ΔPYD, I125M-END) tags (contains a stop codon) |
| 218641 | pDONR221 msNLRP3(ΔPYD, I125M-END) | Gateway shuttling vector for cloning N- and C-terminal NLRP3(ΔPYD, I125M-END) tags (contains no stop codon) |
| 218642 | pLenti UBC hNLRP3-mNG | Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection |
| 218643 | pHAGE pGK hNLRP3-mNG | Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection |
| 218644 | pDONR221 hNLRP3-mNG-STOP | Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon) |
| 218645 | pDONR221 FLAG-APEX2-P4C-IRES-mNeonGreen | Gateway shuttling vector for APEX2-tagged P4C, a P4C biosensor |
| 218647 | pDONR221 hNLRP3 R262W (disease-associated mutation) | Gateway shuttling vector for cloning NLRP3-R262W (no stop codon) |
| 218648 | msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti | Knockout vector for mouse Plekha3 (Fapp1) |
| 218649 | msPlekha3 (msFapp1) g2 lentiCRISPRv2-opti | Knockout vector for mouse Plekha3 (Fapp1) |
| 218650 | msPlekha8 (msFapp2) g1 lentiCRISPRv2-opti | Knockout vector for mouse Plekha8 (Fapp2) |
| 218651 | msPlekha8 (msFapp2) g2 lentiCRISPRv2-opti | Knockout vector for mouse Plekha8 (Fapp2) |
| 218652 | msHook2 g1 lentiCRISPRv2-mCherry | Knockout vector for mouse Hook2 |
| 218653 | msHook2 g2 lentiCRISPRv2-mCherry | Knockout vector for mouse Hook2 |
| 218654 | msFam160b1 (msFHIP2A) g1 lentiCRISPRv2-mCherry | Knockout vector for mouse Fam160b1 (Fhip2A) |
| 218655 | msFam160b1 (msFHIP2A) g2 lentiCRISPRv2 mCherry | Knockout vector for mouse Fam160b1 (Fhip2A) |
| 218656 | PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti | Knockout vector for human PLEKHA3 (FAPP1) |
| 218657 | PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti | Knockout vector for human PLEKHA3 (FAPP1) |
| 218658 | PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti | Knockout vector for human PLEKHA8 (FAPP2) |
| 218659 | PLEKHA8 (FAPP2) g2 lentiCRISPRv2-opti | Knockout vector for human PLEKHA8 (FAPP2) |
| 218660 | TMEM165 g1 LentiCRISPRv2-mCherry | Knockout vector for human TMEM165 |
| 218661 | TMEM165 g2 LentiCRISPRv2-mCherry | Knockout vector for human TMEM165 |
| 218662 | GOLIM4 g1 LentiCRISPRv2-mCherry | Knockout vector for human GOLIM4 (GPP130/GOLPH4) |
| 218663 | GOLIM4 g2 LentiCRISPRv2-mCherry | Knockout vector for human GOLIM4 (GPP130/GOLPH4) |