A single-component, light-assisted uncaging switch for endoproteolytic release.
Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210501 | pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA | expresses LAUNCHER component in mammalian cells |
| 210502 | pCMV-PDGFR-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-tTA | expresses LAUNCHERtvmv component in mammalian cells |
| 210503 | pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA | expresses LAUNCHERsbmv component in mammalian cells |
| 210504 | pCMV-NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry | expresses NES-TvmvC-43LK-iLID-TvmvN-TVMVseq(P1'S)-mCherry component in mammalian cells |
| 210505 | pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP | expresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cells |
| 210506 | pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 | expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cells |
| 210508 | pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c | expresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cells |
| 210509 | hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP | expresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cells |
| 210510 | pAAV-cFOS-FRB-VP16-P2A-EGFP | expresses cFOS-FRB-VP16 and EGFP component in rat hippocampal neuron cells |
| 210511 | TRE-SEAP | SEAP reporter vector for LAUNCHER system |
| 210512 | TRE-tdTomato | tdTomato reporter vector for LAUNCHER system |
| 210513 | pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON)(TRE-SEAP | expresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetON and SEAP reporter in mammalian cells |
| 210514 | DRD2-V2tail-TevN-BLITz-TetR-FKBP | expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian cells |
| 210515 | pCMV-NLS-FRB-VP16 | expresses NLS-FRB-VP16 component in mammalian cells |
| 210516 | pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs | expresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cells |