A programmable reaction-diffusion system for spatiotemporal cell signaling circuit design.
Rajasekaran R, Chang CC, Weix EWZ, Galateo TM, Coyle SM
Cell. 2023 Dec 27:S0092-8674(23)01339-9. doi: 10.1016/j.cell.2023.12.007.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 213112 | pLV-EF1a-mCherry-MinD-IRES-Puro | Expression of mCherry-MinD in mammalian cells |
| 213113 | pLV-EF1a-MinE-EGFP_IRES-Blast | Expression of MinE-EGFP in mammalian cells |
| 213114 | pLV-EF1a-MinD-IRES-Puro | Expression of untagged MinD in mammalian cells |
| 213115 | pLV-EF1a-MinE-IRES-Blast | Expression of untagged MinE in mammalian cells |
| 213116 | pLV-EF1a-FRB-mCherry-MinD-IRES-Puro | Expression of FRB-mCherry-MinD in mammalian cells for payload delivery |
| 213117 | pLV-EF1a-MinE(L4E)-EGFP_IRES-Blast | Expression of a faster-running MinE mutant in mammalian cells |
| 213118 | pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro | Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells |
| 213119 | pTwist_Lenti_SFFV-ActA(1-584)-BFP-FKBP | Expression of an ActA FKBP payload for MinDE recruitment circuits in mammalian cells |