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GTSF1 accelerates target RNA cleavage by PIWI-clade Argonaute proteins.

Arif A, Bailey S, Izumi N, Anzelon TA, Ozata DM, Andersson C, Gainetdinov I, MacRae IJ, Tomari Y, Zamore PD
Nature. 2022 Aug;608(7923):618-625. doi: 10.1038/s41586-022-05009-0. Epub 2022 Jun 30. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
212575pSCALPS-Flag-Miwi-EGFPLentiviral expression of Flag-tagged mouse Miwi in mammalian cells
212576pSCALPS-Flag-Mili-EGFPLentiviral expression of Flag-tagged mouse Mili in mammalian cells
214671pColdI-GST-mGTSF1Bacterial expression of GST-tagged mouse GTSF1
214817pColdI-GST-mGTSF1lBacterial expression of GST-tagged mouse GTSF1l
215714pColdI-GST-mGTSF2Bacterial expression of GST-tagged GTSF2

Antibodies from Article