Visualization of accessible cholesterol using a GRAM domain-based biosensor.

Koh DHZ, Naito T, Na M, Yeap YJ, Rozario P, Zhong FL, Lim KL, Saheki Y
Nat Commun. 2023 Oct 25;14(1):6773. doi: 10.1038/s41467-023-42498-7. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
211701	EGFP-GRAM-W	Expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (EGFP-GRAM1b G187W) in mammalian cells
211702	pLJM1-EGFP-GRAM1b	Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b (pLJM1-EGFP-GRAM1b)
211703	pLJM1-EGFP-GRAM-H	Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187L mutation (pLJM1-EGFP-GRAM1b G187L)
211704	pLJM1-EGFP-GRAM-W	Lentiviral expression of EGFP-tagged GRAM domain of GRAMD1b carrying a G187W mutation (pLJM1-EGFP-GRAM1b G187W)
211705	PM-RA	Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (RA) fused with plasma membrane (PM) targetting motif of Lck in mammalian cells
211706	B-GRAM-H	Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H) in mammalian cells
211707	B-GRAM-W	Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells
211708	pLJM1-PM-RA-BlastR	Lentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)
211709	pLJM1-B-GRAM-H-NeoR	Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187L mutation (B-GRAM-H)
211710	pLJM1-B-GRAM-W-NeoR	Lentiviral expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W)