pLJM1-PM-RA-BlastR
(Plasmid
#211708)
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PurposeLentiviral expression of a ddFP subunit (RA) fused with the lipid modification motif of lymphocyte-specific protein tyrosine kinase (Lck) for plasma membrane (PM) targetting (PM-RA)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211708 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepLJM1
- Total vector size (bp) 7814
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Modifications to backboneReplaced puromycin resistance gene with blasticidin resistance gene
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsNEB® Turbo Competent E. coli can also be used.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePM-targetted RA
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Alt nameLck N-terminal lipidation signal fused with RA
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SpeciesSynthetic
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Insert Size (bp)765
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GenBank IDNM_001162433.2
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Entrez GeneLck (a.k.a. Hck-3, Lsk, Lskt, p56<lck>, p56Lck)
- Promoter CMV
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Tag
/ Fusion Protein
- RA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLJM1-PM-RA-BlastR was a gift from Yasunori Saheki (Addgene plasmid # 211708 ; http://n2t.net/addgene:211708 ; RRID:Addgene_211708) -
For your References section:
Visualization of accessible cholesterol using a GRAM domain-based biosensor. Koh DHZ, Naito T, Na M, Yeap YJ, Rozario P, Zhong FL, Lim KL, Saheki Y. Nat Commun. 2023 Oct 25;14(1):6773. doi: 10.1038/s41467-023-42498-7. 10.1038/s41467-023-42498-7 PubMed 37880244