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Addgene

pLJM1-PM-RA-BlastR
(Plasmid #211708)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211708 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLJM1
  • Total vector size (bp) 7814
  • Modifications to backbone
    Replaced puromycin resistance gene with blasticidin resistance gene
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    NEB® Turbo Competent E. coli can also be used.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PM-targetted RA
  • Alt name
    Lck N-terminal lipidation signal fused with RA
  • Species
    Synthetic
  • Insert Size (bp)
    765
  • GenBank ID
    NM_001162433.2
  • Entrez Gene
    Lck (a.k.a. Hck-3, Lsk, Lskt, p56<lck>, p56Lck)
  • Promoter CMV
  • Tag / Fusion Protein
    • RA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLJM1-PM-RA-BlastR was a gift from Yasunori Saheki (Addgene plasmid # 211708 ; http://n2t.net/addgene:211708 ; RRID:Addgene_211708)
  • For your References section:

    Visualization of accessible cholesterol using a GRAM domain-based biosensor. Koh DHZ, Naito T, Na M, Yeap YJ, Rozario P, Zhong FL, Lim KL, Saheki Y. Nat Commun. 2023 Oct 25;14(1):6773. doi: 10.1038/s41467-023-42498-7. 10.1038/s41467-023-42498-7 PubMed 37880244