Metagenome mining and functional analysis reveal oxidized guanine DNA repair at the Lost City Hydrothermal Field.

Utzman PH, Mays VP, Miller BC, Fairbanks MC, Brazelton WJ, Horvath MP
PLoS One. 2024 May 8;19(5):e0284642. doi: 10.1371/journal.pone.0284642. eCollection 2024. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
210791	pkk223_TtMutY	pkk223 with Thiotrichaceae MutY insert
210792	pkk223_TtMutY_D147N_E46Q	pkk223 with Thiotrichaceae MutY containing D147N and E46Q mutations to reduce catalytic activity
210793	pkk223_TtMutY_F305A_S306A_H307A	pkk223 with Thiotrichaceae MutY containing F305A, S306A, and H307A mutations to reduce recognition of OG:A
210794	pkk223_MsMutY_D150N_E49Q	pkk223 with Marinosulfonomonas MutY containing D150N and E49Q mutations to reduce catalytic activity
210795	pkk223_MsMutY_F302A_T303A_H304A	pkk223 with Marinosulfonomonas MutY containing F302A, T303A, and H304A mutations to reduce recognition of OG:A
210796	pkk223_MsMutY	pkk223 with Marinosulfonomonas MutY insert
210797	pkk223_RbMutY	pkk223 with Rhodobacteraceae MutY insert
210798	pkk223_RbMutY_D146N_E45Q	pkk223 with Rhodobacteraceae MutY containing D146N and E45Q mutations to reduce catalytic activity
210799	pkk223_RbMutY_F298A_T299A_H300A	pkk223 with Rhodobacteraceae MutY containing F298A, S299A, and H300A mutations to reduce recognition of OG:A
213110	pkk223_EcMutY	pkk223 with E coli MutY insert
213111	pkk223_Null	pkk223 backbone plasmid