Visible Light-Induced Specific Protein Reaction Delineates Early Stages of Cell Adhesion.
Rahikainen R, Vester SK, Turkki P, Janosko CP, Deiters A, Hytonen VP, Howarth M
J Am Chem Soc. 2023 Nov 15;145(45):24459-24465. doi: 10.1021/jacs.3c07827. Epub 2023 Oct 31.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 210021 | TfR-SpyCatcher003(K31TAG)-sfGFP-MycTag-CTag | Expression of transferrin receptor transmembrane domain and photocaged SpyCatcher003(K31HCK)-sfGFP on mammalian cell surface. |
| 210022 | SpyCatcher003(K31TAG)-Talin rod-mScarletI | Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells |
| 210023 | EGFP-Talin head-SpyCatcher003(K31TAG) | Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells |
| 210024 | LifeAct-mNeonGreen -IRES- Talin head-SpyTag003 | Expression of LifeAct-mNeonGreen and Talin head(1-433)-SpyTag003 in mammalian cells |
| 210025 | SpyTag003(M115A)-Talin rod-mCherry | Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells |
| 210026 | SpyTag003(M115G)-Talin rod-mCherry | Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells |
| 210027 | SpyTag003(V114T, V116T)-Talin rod-mCherry | Expression of SpyTag003(V114T, V116T)-Talin rod(434-2541)-mCherry in mammalian cells |