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Alternative splicing of its 5' UTR limits CD20 mRNA translation and enables resistance to CD20-directed immunotherapies.

Ang Z, Paruzzo L, Hayer KE, Schmidt C, Torres-Diz M, Xu F, Zankharia U, Zhang Y, Soldan SS, Zheng S, Falkenstein CD, Loftus JP, Yang SY, Asnani M, King Sainos P, Pillai V, Chong ER, Li M, Tasian SK, Barash Y, Lieberman PM, Ruella M, Schuster SJ, Thomas-Tikhonenko A
Blood. 2023 Sep 8:blood.2023020400. doi: 10.1182/blood.2023020400. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
209749LentiCRISPRv2-sgCD20Lentiviral transfer plasmid to express Cas9 and a gRNA targeting the human CD20 gene, MS4A1.
209750LentiCRISPRv2-sgCTRLLentiviral transfer plasmid to express Cas9 and a control, non-specific gRNA (does not target any human gene)
209751pCDH-EF1-IRES-BSDLentiviral transfer plasmid to express the blasticidin resistance gene
209752pCDH-V1-rCD20-BSDTo reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.
209753pCDH-V2-rCD20-BSDTo reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.
209754pCDH-V3-rCD20-BSDTo reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.
209755pCDH-V1-CD20-PuroLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 1 mRNA isoform.
209756pCDH-V2-CD20-PuroLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.
209757pCDH-V3-CD20-PuroLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 3 mRNA isoform.
209758pCDH-V1AUC-DelStem-CD20-PuroLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.
209759pCDH-kz-CD20-PuroLentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

Antibodies from Article