Rapid iPSC inclusionopathy models shed light on formation, consequence and molecular subtype of alpha-synuclein inclusions
Lam I, Ndayisaba A, Lewis AJ, Fu Y, Sagredo GT, Zaccagnini L, Sandoe J, Sanz RL, Vahdatshoar A, Martin TD, Morshed N, Ichihashi T, Tripathi A, Ramalingam N, Oettgen-Suazo C, Bartels T, Schäbinger M, Hallacli E, Jiang X, Verma A, Tea C, Wang Z, Hakozaki H, Yu X, Hyles K, Park C, Theunissen TW, Wang H, Jaenisch R, Lindquist S, Stevens B, Stefanova N, Wenning G, Luk KC, Sanchez Pernaute R, Gómez-Esteban JC, Felsky D, Kiyota Y, Sahni N, Yi SS, Chung C, Stahlberg H, Ferrer I, Schöneberg J, Elledge SJ, Dettmer U, Halliday GM, Bartels T, Khurana V
bioRxiv 2022.11.08.515615
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 209078 | piggyBac-rtTA (4th_Gen)-SNCA(3K)-sfGFP-IRES-NGN2-puro (VK_1120) | Ngn2 mediated cortical neuron induction, along with SNCA(3K) over-expression |
| 209079 | piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122) | Ngn2 mediated cortical neuron induction, along with sfGFP over-expression |
| 209080 | piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123) | Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression |
| 209081 | piggyBac-rtTA (4th_Gen)-SNCA(A53T-DNAC)-sfGFP-IRES-NGN2-puro (VK_1124) | Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-DeltaNAC-sfGFP over-expression |
| 209082 | piggyBac-rtTA (4th_Gen)-NFIB-SOX9-IRES-mKate2 (VK_1244) | NFIB and SOX9 mediated astrocye induction |