qTAG: an adaptable plasmid scaffold for CRISPR-based endogenous tagging.
Philip R, Sharma A, Matellan L, Erpf AC, Hsu WH, Tkach JM, Wyatt HDM, Pelletier L
EMBO J. 2024 Dec 12. doi: 10.1038/s44318-024-00337-5.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
207676 | qTAG-N-Blast-mNeon | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette |
207677 | qTAG-N-Blast-moxGFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette |
207678 | qTAG-N-Blast-mScarlet | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette |
207679 | qTAG-N-Blast-sTagRFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette |
207680 | qTAG-N-Blast-miRFP670nano3 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassette |
207681 | qTAG-N-Blast-miniTurbo | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miniTurbo cassette |
207682 | qTAG-N-Blast-ultraID | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette |
207683 | qTAG-N-Blast-dTAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette |
207684 | qTAG-N-Blast-3xFLAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xFLAG cassette |
207685 | qTAG-N-Blast-3xHA | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassette |
207686 | qTAG-N-Blast-V5 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-V5 cassette |
207687 | qTAG-N-Puro-mNeon | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette |
207688 | qTAG-N-Puro-moxGFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassette |
207689 | qTAG-N-Puro-mScarlet | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mScarlet cassette |
207690 | qTAG-N-Puro-sTagRFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-sTagRFP cassette |
207691 | qTAG-N-Puro-miRFP670nano3 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassette |
207692 | qTAG-N-Puro-miniTurbo | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miniTurbo cassette |
207693 | qTAG-N-Puro-ultraID | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette |
207694 | qTAG-N-Puro-dTAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette |
207695 | qTAG-N-Puro-3xFLAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xFLAG cassette |
207696 | qTAG-N-Puro-3xHA | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette |
207697 | qTAG-N-Puro-V5 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette |
207698 | qTAG-N-Zeo-mNeon | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette |
207699 | qTAG-N-Zeo-moxGFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-moxGFP cassette |
207700 | qTAG-N-Zeo-mScarlet | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mScarlet cassette |
207701 | qTAG-N-Zeo-sTagRFP | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-sTagRFP cassette |
207702 | qTAG-N-Zeo-miRFP670nano3 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miRFP670nano3 cassette |
207703 | qTAG-N-Zeo-miniTurbo | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassette |
207704 | qTAG-N-Zeo-ultraID | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette |
207705 | qTAG-N-Zeo-dTAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-dTAG cassette |
207706 | qTAG-N-Zeo-3xFLAG | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette |
207707 | qTAG-N-Zeo-3xHA | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xHA cassette |
207708 | qTAG-N-Zeo-V5 | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-V5 cassette |
207709 | qTAG-C-mNeon-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette |
207710 | qTAG-C-moxGFP-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Blast cassette |
207711 | qTAG-C-mScarlet-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette |
207712 | qTAG-C-sTagRFP-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Blast cassette |
207713 | qTAG-C-miRFP670nano3-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette |
207714 | qTAG-C-miniTurbo-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassette |
207715 | qTAG-C-ultraID-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette |
207716 | qTAG-C-dTAG-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Blast cassette |
207717 | qTAG-C-3xFLAG-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette |
207718 | qTAG-C-3xHA-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Blast cassette |
207719 | qTAG-C-V5-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette |
207720 | qTAG-C-mNeon-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Puro cassette |
207721 | qTAG-C-moxGFP-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Puro cassette |
207722 | qTAG-C-mScarlet-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette |
207723 | qTAG-C-sTagRFP-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Puro cassette |
207724 | qTAG-C-miRFP670nano3-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette |
207725 | qTAG-C-miniTurbo-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Puro cassette |
207726 | qTAG-C-ultraID-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Puro cassette |
207727 | qTAG-C-dTAG-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Puro cassette |
207728 | qTAG-C-3xFLAG-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Puro cassette |
207729 | qTAG-C-3xHA-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Puro cassette |
207730 | qTAG-C-V5-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette |
207731 | qTAG-C-mNeon-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette |
207732 | qTAG-C-moxGFP-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassette |
207733 | qTAG-C-mScarlet-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette |
207734 | qTAG-C-sTagRFP-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a sTagRFP-2A-Zeo cassette |
207735 | qTAG-C-miRFP670nano3-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Zeo cassette |
207736 | qTAG-C-miniTurbo-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Zeo cassette |
207737 | qTAG-C-ultraID-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassette |
207738 | qTAG-C-dTAG-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette |
207739 | qTAG-C-3xFLAG-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette |
207740 | qTAG-C-3xHA-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xHA-2A-Zeo cassette |
207741 | qTAG-C-V5-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette |
207742 | pEF1α-Cre-PGK-Puro | Co-expression of the Cre recombinase and a puro selectable marker |
207743 | pEF1α-Cre-2A-Puro | Expression of the Cre recombinase linked to a puro selectable marker |
207744 | pEF1α-Cre-2A-moxGFP | Expression of the Cre recombinase linked to a moxGFP fluorescent marker |
207745 | pEF1α-Cre-2A-mScarlet | Expression of the Cre recombinase linked to a mScarlet fluorescent marker |
207746 | pEF1α-Cre-2A-mTagBFP2 | Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker |
207747 | pEF1α-Cre-2A-miRFP670nano3 | Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker |
207748 | pX330-PITCh-ACTB | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in. |
207749 | qTAG-N-Solo-mNeon-ACTB | Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
207750 | qTAG-N-Solo-mScarlet-ACTB | Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
207751 | qTAG-N-Blast-mNeon-ACTB | Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
207752 | qTAG-N-Puro-mNeon-ACTB | Donor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
207753 | qTAG-N-Blast-mScarlet-ACTB | Donor template for Blast-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
207754 | qTAG-N-Puro-mScarlet-ACTB | Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748 |
207755 | pX330-PITCh-H2BC11 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in. |
207756 | qTAG-C-Solo-mScarlet-H2BC11 | Donor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207757 | qTAG-C-moxGFP-Blast-H2BC11 | Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207758 | qTAG-C-moxGFP-Puro-H2BC11 | Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207759 | qTAG-C-moxGFP-Zeo-H2BC11 | Donor template for moxGFP-2A-Zeo insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207760 | qTAG-C-moxGFP-Puro-H2BC11-MMEJ | MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207761 | qTAG-C-mScarlet-Puro-H2BC11 | Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207762 | qTAG-C-miRFP670nano3-Blast-H2BC11 | Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755 |
207763 | pX330-PITCh-TUBA1B | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in. |
207764 | qTAG-N-Solo-mScarlet-TUBA1B | Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
207765 | qTAG-N-Solo-sTagRFP-TUBA1B | Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
207766 | qTAG-N-Blast-moxGFP-TUBA1B | Donor template for Blast-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
207767 | qTAG-N-Puro-moxGFP-TUBA1B | Donor template for Puro-2A-moxGFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
207768 | qTAG-N-Blast-sTagRFP-TUBA1B | Donor template for Blast-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
207769 | qTAG-N-Puro-sTagRFP-TUBA1B | Donor template for Puro-2A-sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763 |
207770 | pX330-PITCh-LMNB1 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in. |
207771 | qTAG-N-Solo-mScarlet-LMNB1 | Donor template for mScarlet insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207772 | qTAG-N-Blast-moxGFP-LMNB1 | Donor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207773 | qTAG-N-Puro-moxGFP-LMNB1 | Donor template for Puro-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207774 | qTAG-N-Blast-miniTurbo-LMNB1 | Donor template for Blast-2A-miniTurbo insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207775 | qTAG-N-Blast-ultraID-LMNB1 | Donor template for Blast-2A-ultraID insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207776 | qTAG-N-Blast-dTAG-LMNB1 | Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207777 | qTAG-N-Blast-3xFLAG-LMNB1 | Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207778 | qTAG-N-Blast-3xHA-LMNB1 | Donor template for Blast-2A-3xHA insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207779 | qTAG-N-Blast-V5-LMNB1 | Donor template for Blast-2A-V5 insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770 |
207780 | pX330-PITCh-H3C2 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H3C2 for knock-in. |
207781 | qTAG-C-Solo-mScarlet-H3C2 | Donor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
207782 | qTAG-C-mNeon-Blast-H3C2 | Donor template for mNeon-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
207783 | qTAG-C-moxGFP-Puro-H3C2 | Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
207784 | qTAG-C-miRFP670nano3-Blast-H3C2 | Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780 |
207785 | pX330-PITCh-TUBB4B | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TUBB4B for knock-in. |
207786 | qTAG-C-mNeon-Blast-TUBB4B | Donor template for mNeon-2A-Blast insertion into the C-terminus of the TUBB4B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBB4B Addgene #207785 |
207787 | pX330-LAMP1 | Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in. |
207788 | qTAG-C-moxGFP-Puro-LAMP1 | Donor template for moxGFP-2A-Puro insertion into the C-terminus of the LAMP1 locus. For lysosome visualization.To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787) |
207789 | pX330-PITCh-TOMM20 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of TOMM20 for knock-in. |
207790 | qTAG-C-mNeon-Puro-TOMM20 | Donor template for mNeon-2A-Puro insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789) |
207791 | pX330-PITCh-GOLGA2 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in. |
207792 | qTAG-N-Puro-moxGFP-GOLGA2 | Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791 |
207793 | pX330-PITCh-MAPRE1 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of MAPRE1 for knock-in. |
207794 | qTAG-C-moxGFP-Puro-MAPRE1 | Donor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793 |
227250 | qTAG-N-Puro-mStayGold | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette |
227251 | qTAG-N-Blast-mStayGold | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mStayGold cassette |
227252 | qTAG-N-Zeo-mStayGold | Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette |
227253 | qTAG-C-mStayGold-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Puro cassette |
227254 | qTAG-C-mStayGold-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette |
227255 | qTAG-C-mStayGold-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette |
227256 | qTAG-C-mStayGold-EFS-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette |
227257 | qTAG-C-mStayGold-EFS-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette |
227258 | qTAG-C-mStayGold-EFS-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Zeo cassette |
227259 | qTAG-C-mScarlet-EFS-Puro | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassette |
227260 | qTAG-C-mScarlet-EFS-Blast | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassette |
227261 | qTAG-C-mScarlet-EFS-Zeo | Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassette |
227262 | qTAG-KO-Puro | Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Puro cassette |
227263 | qTAG-KO-Blast | Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a 2A-Blast cassette |
227264 | qTAG-KO-PGK-Puro | Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette |
227265 | qTAG-KO-PGK-Blast | Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Blast cassette |
227266 | qTAG-AAVS1-Ef1a-Puro | Empty AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site. |
227267 | qTAG-AAVS1-Ef1a-Blast | Empty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site. |
227268 | qTAG-AAVS1-Ef1a-Zeo | Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site. |
227269 | qTAG-AAVS1-PGK-Puro | Empty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site. |
227270 | qTAG-AAVS1-PGK-Blast | Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site. |
227271 | qTAG-AAVS1-PGK-Zeo | Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site. |
227272 | pX330-AAVS1 | Expresses SpCas9 and a sgRNA targeting the AAVS1 loci for knock-in. |
227273 | qTAG-AAVS1-Ef1a-Puro-moxGFP | AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272) |
227274 | qTAG-AAVS1-Ef1a-Puro-mScarlet | AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272) |
227275 | qTAG-AAVS1-PGK-Puro-moxGFP | AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272) |
227276 | pX330-PITCh-ARL13B | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of ARL13B for knock-in. |
227277 | qTAG-C-Solo-mStayGold-ARL13B | Donor template for mStayGold insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with sgRNA plasmid pX330-PITCh-ARL13B (Addgene #227276) |
227278 | qTAG-C-mStayGold-EFS-Blast-ARL13B | Donor template for mStayGold-EFS-Blast insertion into the C-terminus of the ARL13B locus. For cilia visualization. To be co-transfected with plasmid pX330-PITCh-ARL13B (Addgene #227276) |
227279 | pX330-CANX | Expresses SpCas9 and a sgRNA targeting the C-terminus of CANX for knock-in. |
227280 | qTAG-C-Solo-mStayGold-CANX | Donor template for mStayGold insertion into the C-terminus of the CANX locus. For ER visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279) |
227281 | qTAG-C-mStayGold-Puro-CANX | Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CANX locus. For cilia visualization. To be co-transfected with sgRNA plasmid px330-CANX (Addgene #227279) |
227282 | pX330-CENPA | Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in. |
227283 | qTAG-N-Solo-mStayGold-CENPA | Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282) |
227284 | pX330-PCNT | Expresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in. |
227285 | qTAG-N-Solo-mStayGold-PCNT | Donor template for mStayGold insertion into the N-terminus of the PCNT locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PCNT (Addgene #227284) |
227286 | pX330-CEP135 | Expresses SpCas9 and a sgRNA targeting the N-terminus of CEP135 for knock-in. |
227287 | qTAG-N-Solo-mStayGold-CEP135 | Donor template for mStayGold insertion into the N-terminus of the CEP135 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CEP135 (Addgene #227286) |
227288 | pX330-PITCh-CEP192 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CEP192 for knock-in. |
227289 | qTAG-C-Solo-mStayGold-CEP192 | Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288) |
227290 | qTAG-C-mStayGold-EFS-Puro-CEP192 | Donor template for mStayGold-EFS-Puro insertion into the C-term of CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288) |
227291 | pX330-CETN2 | Expresses SpCas9 and a sgRNA targeting the N-terminus of CETN2 for knock-in. |
227292 | qTAG-N-Solo-mStayGold-CETN2 | Donor template for mStayGold insertion into the N-terminus of the CETN2 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px330-CETN2 (Addgene #227291) |
227293 | pX330-EZR | Expresses SpCas9 and a sgRNA targeting the C-terminus of EZR for knock-in. |
227294 | qTAG-C-Solo-mStayGold-EZR | Donor template for mStayGold insertion into the C-terminus of the EZR locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-EZR (Addgene #227293) |
227295 | pX330-PITCh-MAP4 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MAP4 for knock-in. |
227296 | qTAG-N-Solo-mStayGold-MAP4 | Donor template for mStayGold insertion into the N-terminus of the MAP4 locus. For microtubule visualization. To be co-transfected with sgRNAplasmid px330-PITCh-MAP4 (Addgene #227295) |
227297 | pX330-RAB7A | Expresses SpCas9 and a sgRNA targeting the N-terminus of RAB7A for knock-in. |
227298 | qTAG-N-Solo-mStayGold-RAB7A | Donor template for mStayGold insertion into the N-terminus of the RAB7A locus. For endosome visualization. To be co-transfected with sgRNA plasmid px330-RAB7A (Addgene #227297) |
227299 | pX330-TJP1 | Expresses SpCas9 and a sgRNA targeting the N-terminus of TJP1 for knock-in. |
227300 | qTAG-N-Solo-mStayGold-TJP1 | Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299) |
227301 | pX330-PITCh-VIM | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in. |
227302 | qTAG-C-Solo-mStayGold-VIM | Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301) |
227303 | pX330-PITCh-PEX3 | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PEX3 for knock-in. |
227304 | qTAG-C-Solo-mStayGold-PEX3 | Donor template for mStayGold insertion into the C-terminus of the PEX3 locus. For peroxisome visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PEX3 (Addgene #227303) |
227305 | pX330-PITCh-MYO1C | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in. |
227306 | qTAG-N-Solo-mStayGold-MYO1C | Donor template for mStayGold insertion into the N-terminus of the MYO1C locus. For membrane visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MYO1C (Addgene #227305) |
227307 | qTAG-C-Solo-mStayGold-TOMM20 | Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789) |
227308 | pX330-PITCh-COX8A | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of COX8A for knock-in. |
227309 | qTAG-C-Solo-mStayGold-COX8A | Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308) |
227310 | pX458-PLK4 | Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in. |
227311 | qTAG-C-mStayGold-EFS-Puro-PLK4 | Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310) |
227312 | pX330-PITCh-CLTC | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of CLTC for knock-in. |
227313 | qTAG-C-Solo-mStayGold-CLTC | Donor template for mStayGold insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312) |
227314 | qTAG-C-mStayGold-Puro-CLTC | Donor template for mStayGold-2A-Puro insertion into the C-terminus of the CLTC locus. For clathrin heavy chain visualization. To be co-transfected with sgRNA px330-PITCh-CLTC (Addgene #227312) |
227315 | pX330-PITCh-PXN | Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of PXN for knock-in. |
227316 | qTAG-C-Solo-mStayGold-PXN | Donor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315) |
227317 | qTAG-C-mStayGold-Puro-PXN | Donor template for mStayGold-2A-Puro insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315) |
227318 | pX330-TP53 | Expresses SpCas9 and a sgRNA targeting the N-terminus of TP53 for knock-in. |
227319 | qTAG-KO-Puro-TP53 | Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318) |
227320 | qTAG-N-Puro-mStayGold-ACTB | Donor template for Puro-2A-mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748) |
227321 | qTAG-N-Puro-mStayGold-TUBA1B | Donor template for Puro-2A-mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763) |
227322 | qTAG-C-Solo-mStayGold-LAMP1 | Donor template for mStayGold insertion into the C-terminus of the LAMP1 locus. For lysosome visualization. To be co-transfected with sgRNA plasmid px330-LAMP1 (Addgene #207787) |
227323 | qTAG-C-Solo-mScarlet-MAPRE1 | Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793) |
227324 | qTAG-C-Solo-mStayGold-MAPRE1 | Donor template for mStayGold insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793) |
227325 | qTAG-N-Solo-mStayGold-GOLGA2 | Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791) |
227326 | qTAG-N-Solo-mStayGold-ACTB | Donor template for mStayGold insertion into the N-terminus of the ACTB locus. For actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB (Addgene #207748) |
227327 | qTAG-N-Solo-mStayGold-TUBA1B | Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763) |
227328 | qTAG-N-Solo-mStayGold-LMNB1 | Donor template for mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770) |
227329 | qTAG-N-Puro-mStayGold-LMNB1 | Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770) |
227330 | qTAG-C-Solo-mStayGold-H2BC11 | Donor template for mStayGold insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755) |
227331 | qTAG-C-Solo-miRFP670nano3-H2BC11 | Donor template for miRFP670nano3 insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755) |
227332 | qTAG-C-mStayGold-Puro-H2BC11 | Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755) |
227333 | qTAG-C-mStayGold-Puro-H2BC11-MMEJ | MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755) |
227334 | qTAG-C-Solo-mStayGold-H3C2 | Donor template for mStayGold insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780) |
227335 | qTAG-C-Solo-miRFP670nano3-H3C2 | Donor template for miRFP670nano3 insertion into the C-terminus of the H3C2 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 (Addgene #207780) |