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Frank von Delft Lab: The SARS-Cov-2 main protease (Mpro) is responsible for viral polyprotein processing and therefore a therapeutic target.

Unpublished

Plasmids from Article

ID Plasmid Purpose
204769SARS-CoV-2 Mpro protease (C145S mutant - catalytically inactive); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO C145S mutant
204770SARS-CoV-2 Mpro protease (R188K mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO R188K mutant
204771SARS-CoV-2 Mpro protease (A193S mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO A193S mutant
204772SARS-CoV-2 Mpro protease (A191T mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO A191T mutant
204773SARS-CoV-2 Mpro protease (A193T mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO A193T mutant
204774SARS-CoV-2 Mpro protease (A191V mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO A191V mutant
204776SARS-CoV-2 Mpro protease (G143S mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO G143S mutant
204777SARS-CoV-2 Mpro protease (M491I mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO M49I mutant
204778SARS-CoV-2 Mpro protease (M165I mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO M165I mutant
204779SARS-CoV-2 Mpro protease (M49T mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO M49T mutant
204782SARS-CoV-2 Mpro protease (N142S mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO N142S mutant
204783SARS-CoV-2 Mpro protease (P132H mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO P132H mutant
204784SARS-CoV-2 Mpro protease (Q189K mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO Q189K mutant
204785Sars Cov 2 MPRO R188S mutantSARS-CoV-2 Mpro protease (R188S mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO R188S mutant
204786SARS-CoV-2 Mpro protease (T190I mutant - clinical mutant); aliases: 3CLpro, 3C-likeBacterial expression of Sars Cov 2 MPRO T190I mutant
204787Sars Cov 2 Mac domainBacterial expression of Sars Cov 2 Mac domain
204788Zika NS5 RdRpBacterial expression of Zika NS5 RdRp
204790MERS Mpro - His6-SUMO with autoprocessing - MSAVMQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWLBacterial expression of MERS Mpro
204791ZIKV NS2B-NS3 protease (H115A- catalytically inactive); aliases: Zika Virus NS2B-NS3 fusion protein expressed with N-terminal TwBacterial expression of a fusion of Zika NS2B-NS3 proteins
204792Fusion of Dengue2 NS2B-NS3 proteinsBacterial expression of a fusion of Dengue2 NS2B-NS3 proteins
204793Fusion of Dengue2 NS2B-NS3 proteinsBacterial expression of a fusion of Dengue2 NS2B-NS3 proteins
204794WNV NS2B-NS3 protease (K104A - catalytically active, don't self cleave); aliases: West Nile Virus NS2B-NS3 fusion proteinBacterial expression of a fusion of West Nile NS2B-NS3 proteins
204795WNV NS2B-NS3 protease (catalytically active, self cleave); aliases: West Nile Virus NS2B-NS3 fusion proteinBacterial expression of a fusion of West Nile NS2B-NS3 proteins
204796Fusion of West Nile NS2B-NS3 proteinsBacterial expression of a fusion of West Nile NS2B-NS3 proteins
204797WNV NS2B-NS3 protease (based on PDB 2GGC, H106A - catalytically inactive); aliases: West Nile Virus NS2B-NS3 fusion proteinBacterial expression of a fusion of West Nile NS2B-NS3 proteins
204798WNV NS2B-NS3 protease (based on PDB 2YOL, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion proteinBacterial expression of a fusion of West Nile NS2B-NS3 proteins
204799WNV NS2B-NS3 protease (based on PDB 2IJO, catalytically active); aliases: West Nile Virus NS2B-NS3 fusion proteinBacterial expression of a fusion of West Nile NS2B-NS3 proteins
204800Fusion of Dengue2 NS2B-NS3Bacterial expression of a fusion of Dengue2 NS2B-NS3 proteins
204801EV-D68 2A protease; aliases: Enterovirus D68 2A protease, 6xHis-tag, low expression levelsBacterial expresison of Enterovirus D68 2A protease
204802EV-D68 2A protease; 6xHis-SUMO, low expression levelsBacterial expresison of Enterovirus D68 2A protease
204803EV-D68 2A protease; 6xHis-MBP-TEV tag, low expression levelsBacterial expresison of Enterovirus D68 2A protease
204804EV-D68 2A protease; 6xHis-GST-TEV, low expression levelsBacterial expresison of Enterovirus D68 2A protease
204805EV-D68 2A protease; no tag, low expression levelsBacterial expresison of Enterovirus D68 2A protease
204806WNV NS2B-NS3 protease: Co-expression of NS2B and NS3Bacterial co-expression of West Nile NS2B and NS3 proteins
204808DENV2 NS2B-NS3 protease: Co-expression of DENV-2 NS2B and NS3Bacterial co-expression of Dengue2 NS2B and NS3 proteins
204809EV-A71 2A protease; 6xHis-tag, self cleavingBacterial expresison of Enterovirus A71 2A protease
204810EV-A71 2A protease; aliases: Enterovirus EV71 2A protease, 6xHis-SUMO-TEV tag, self cleavingBacterial expresison of Enterovirus A71 2A protease
204811EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleavingBacterial expresison of Enterovirus A71 2A protease
204812Enterovirus A71 2A proteaseBacterial expresison of Enterovirus A71 2A protease
204813EV-A71 2A protease; no tag, self cleavingBacterial expresison of Enterovirus A71 2A protease
204814MERS Mpro - His6-SUMO with autoprocessing - MSAVLQ/SGLVKM---MGVVMQ/SGVRKVTYGTAHWLBacterial expression of MERS Mpro
204815Fusion of Zika NS2B-NS3 proteinsBacterial expression of a fusion of Zika NS2B-NS3 proteins
204816EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tagBacterial expresison of Enterovirus A71 3C protease
204817EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tagBacterial expresison of Enterovirus D68 3C protease

Antibodies from Article