miR-27b targets MAIP1 to mediate lipid accumulation in cultured human and mouse hepatic cells.
Sakai E, Imaizumi T, Suzuki R, Taracena-Gandara M, Fujimoto T, Sakurai F, Mizuguchi H
Commun Biol. 2023 Jun 24;6(1):669. doi: 10.1038/s42003-023-05049-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203597 | CSII-TK-MCS | Empty lentiviral vector to express protein in mammalian cells cells. |
| 203598 | CSII-TK-B4GALT3 | Lentiviral vector to express B4GALT3 in mammalian cells |
| 203599 | CSII-TK-MAIP1 | Lentiviral vector to express MAIP1 in mammalian cells |
| 203600 | psicheck2-27bT | Reporter plasmid carrying miR-27b target sequence |
| 203601 | psicheck2-B4GALT3 | Reporter plasmid carrying B4GALT3 3'UTR |
| 203602 | psicheck2-B4GALT3mut | Reporter plasmid carrying B4GALT3 3'UTR with mutated miR27b target sequences. |
| 203603 | psicheck2-MAIP1 | Reporter plasmid carrying MAIP1 3'UTR |
| 203604 | psicheck2-MAIP1mut | Reporter plasmid carrying MAIP1 3'UTR with mutated miR27b target sequences |
| 203605 | psicheck2-PHLPP2 | Reporter plasmid carrying PHLPP2 3'UTR |
| 203606 | psicheck2-PHLPP2mut | Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences |