An enzyme-coupled microplate assay for activity and inhibition of hmdUMP hydrolysis by DNPH1.
Wagner AG, Eskandari R, Schramm VL
Anal Biochem. 2023 Jul 1;672:115171. doi: 10.1016/j.ab.2023.115171. Epub 2023 May 3.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203224 | His-DNPH1 | For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag. |
| 203225 | His-DERA | For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag. |
| 203226 | His-AgdNK | For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag. |