A conserved C-terminal RXG motif in the NgBR subunit of cis-prenyltransferase is critical for prenyltransferase activity.
Grabinska KA, Edani BH, Park EJ, Kraehling JR, Sessa WC
J Biol Chem. 2017 Oct 20;292(42):17351-17361. doi: 10.1074/jbc.M117.806034. Epub 2017 Aug 23.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
203173 | pKGmDUET-NgBR/hCIT | Expression of human cis-prenyltransferase complex for purification |
203174 | pKG-GW1-MaUPPS-A | Yeast expression vector for catalytic subunit of Methanosarcina acetivorans cis-prenyltransferase |
203175 | pKG-GW2-MaUPPS-B | Yeast expression vector for accessory subunit of Methanosarcina acetivorans cis-prenyltransferase |