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A novel homeostatic mechanism tunes PI(4,5)P2-dependent signaling at the plasma membrane.

Wills RC, Doyle CP, Zewe JP, Pacheco J, Hansen SD, Hammond GRV
J Cell Sci. 2023 Aug 3:jcs.261494. doi: 10.1242/jcs.261494. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
202720EGFP-PIP5K1Aexpresses fluorescent protein-tagged enzyme
202722EGFP-PIP5K1Bexpresses fluorescent protein-tagged enzyme
202723EGFP-PIP5K1BD266Kexpresses fluorescent protein-tagged enzyme
202724TagBFP2-Mss4-Kinaexpresses fluorescent protein-tagged enzyme
202725TagBFP2-Mss4D636K-Kinaexpresses fluorescent protein-tagged enzyme
202726TagBFP2-PIP4K2Aexpresses fluorescent protein-tagged enzyme
202727TagBFP2-PIP4K2AD273Kexpresses fluorescent protein-tagged enzyme
202728TagBFP2-PIP4K2Bexpresses fluorescent protein-tagged enzyme
202729TagBFP2- PIP4K2BD278Kexpresses fluorescent protein-tagged enzyme
202730TagBFP2-PIP4K2Cexpresses fluorescent protein-tagged enzyme
202731TagBFP2- PIP4K2CD280Kexpresses fluorescent protein-tagged enzyme
202732mCherry-FKBP-PIP4K2Aexpresses recruitable fluorescent protein-tagged enzyme
202733mCherry-FKBP-PIP4K2AD273Kexpresses recruitable fluorescent protein-tagged enzyme
202734mCherry-FKBP-PIP4K2Bexpresses recruitable fluorescent protein-tagged enzyme
202735mCherry-FKBP-PIP4K2BD278Kexpresses recruitable fluorescent protein-tagged enzyme
202736mCherry-FKBP-PIP4K2Cexpresses recruitable fluorescent protein-tagged enzyme
202737mCherry-FKBP-PIP4K2CD280Kexpresses recruitable fluorescent protein-tagged enzyme
202738TagBFP2-FKBP-INPP5ED477Nexpresses recruitable fluorescent protein-tagged enzyme
202739mCherry-Mss4-Kinaexpresses fluorescent protein-tagged enzyme
202740mCherry-Mss4D636K-Kinaexpresses fluorescent protein-tagged enzyme
202741TagBFP2-FKBP-PIP5K1C_Kina-HDexpresses recruitable fluorescent protein-tagged enzyme
202742TagBFP2-FKBP-PIP5K1C_Kina-HDD316Kexpresses recruitable fluorescent protein-tagged enzyme
202743iRFP713-FRB-PIP5K1Aexpresses recruitable fluorescent protein-tagged enzyme
202744iRFP713-FRB-Mss4-Kinaexpresses recruitable fluorescent protein-tagged enzyme
202745mCherry-FKBP-PIP4K2A-Kinaexpresses recruitable fluorescent protein-tagged enzyme
202746EGFP-INPP5E-CAAXexpress plasma membrane targeted fluorescent protein-tagged enzyme
202747Lyn11C3S-EGFPweakly plasma membrane targeted fluorescent protein
202748EGFP-Lyn11C32S-PIP4K2Aweakly plasma membrane targeted fluorescent protein-tagged enzyme
202749iRFP670-PIP5K1Aexpresses fluorescent protein-tagged enzyme
202750iRFP670-PIP4K2Cexpresses fluorescent protein-tagged enzyme
202751PH-PLCd1-mNGx1expresses fluorescent protein-tagged lipid biosensor
202752PH-PLCd1-mNGx2expresses fluorescent protein-tagged lipid biosensor
202753PH-PLCd1-mNGx3expresses fluorescent protein-tagged lipid biosensor

Antibodies from Article