Diacylglycerol kinase zeta interacts with sphingomyelin synthase 1 and sphingomyelin synthase-related protein via different regions.
Furuta M, Murakami C, Numagami Y, Suzuki R, Sakane F
FEBS Open Bio. 2023 May 11. doi: 10.1002/2211-5463.13628.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202526 | pCAGGS-N-TEV-Twin-Strep | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell |
| 202527 | pCAGGS-C-TEV-Twin-Strep | Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in mammalian cell |
| 202528 | pCAGGS-SMS1-TEV-Twin-Strep | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell |
| 202529 | pCAGGS-SMS2-TEV-Twin-Strep | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase 2 (SMS2) in mammalian cell |
| 202530 | pCAGGS-SMSr-TEV-Twin-Strep | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell |
| 202531 | pCAGGS-Twin-Strep-TEV-DGKζ | Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human diacylglycerol kinase ζ (DGKζ) in mammalian cell |
| 202557 | pCAGGS-SMS1-TEV-HAT | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell |
| 202597 | pCAGGS-C-TEV-HAT | Express N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell |
| 202598 | pCAGGS-N-TEV-HAT | Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cell |
| 203323 | pOET3-N-TEV-Twin-Strep | Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells |
| 203324 | pOET3-C-TEV-Twin-Strep | Generate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells |
| 203325 | pCAGGS-AcGFP-C | Express N-terminal AcGFP-fused protein in mammalian cell |
| 203326 | pCAGGS-mCherry-C | Express N-terminal mCherry-fused protein in mammalian cell |