A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas.
Cervia LD, Shibue T, Borah AA, Gaeta B, He L, Leung L, Li N, Moyer SM, Shim BH, Dumont N, Gonzalez A, Bick NR, Kazachkova M, Dempster JM, Krill-Burger JM, Piccioni F, Udeshi ND, Olive ME, Carr SA, Root DE, McFarland JM, Vazquez F, Hahn WC
Cancer Discov. 2023 Mar 1;13(3):766-795. doi: 10.1158/2159-8290.CD-22-1230.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 202434 | pLX_314-HRI-V5 | Expression of HRI |
| 202435 | pTRE-HRI-V5-IRES-BFP | Lentiviral vector expression of HRI and TagBFP |
| 202436 | pLX209-neo-HA-Ub-T2A-Tet3G | Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G) |
| 202437 | pXPR_003-sgBIRC6-1 | Expression of a guide RNA targeting BIRC6 |
| 202438 | pXPR_003-sgBIRC6-4 | Expression of a guide RNA targeting BIRC6 |
| 202439 | pXPR_003-sgBIRC6-5 | Expression of a guide RNA targeting BIRC6 |
| 202440 | pXPR_003-sgUBA6 | Expression of a guide RNA targeting UBA6 |
| 202441 | pXPR_003-sgKCMF1 | Expression of a guide RNA targeting KCMF1 |
| 202442 | pXPR_003-sgUBR4 | Expression of a guide RNA targeting UBR4 |
| 202443 | pXPR_016-sgHRI | Expression of a guide RNA targeting HRI |
| 202444 | pXPR_023d-sgCiCh2-2 | Expression of a CRISPRi control sgRNA that cuts an intergenic region on chromosome 2 |
| 202445 | pXPR_023d-sgCiPOLR2D | Expression of a CRISPRi guide targeting POLR2D |
| 202446 | pXPR_023d-sgCiSF3B1 | Expression of a CRISPRi guide targeting SF3B1 |
| 202447 | pXPR_023d-sgCiBIRC6-1 | Expression of a CRISPRi guide targeting BIRC6 |
| 202448 | pXPR_023d-sgCiBIRC6-5 | Expression of a CRISPRi guide targeting BIRC6 |
| 202449 | pRSITEP-shBIRC6-2 | DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6 |
| 202450 | pRSITEP-shBIRC6-2-C911 | a seed-matched control for pRSITEP-puro-shBIRC6-2 |
| 202451 | pRSITEP-shBIRC6-2(3) | DOX-inducible expression of a short-hairpin RNA targetinging human BIRC6 |
| 202452 | pRSITEP-shBIRC6-2(3)-C911 | a seed-matched control for pRSITEP-puro-shBIRC6-2(3) |
| 202455 | pXPR_016-sgATF4-1 | Expression of a guide RNA targeting ATF4 |
| 202456 | pXPR_016-sgATF4-3 | Expression of a guide RNA targeting ATF4 |