A bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits.
Wietek J, Nozownik A, Pulin M, Saraf-Sinik I, Matosevich N, Gowrishankar R, Gat A, Malan D, Brown BJ, Dine J, Imambocus BN, Levy R, Sauter K, Litvin A, Regev N, Subramaniam S, Abrera K, Summarli D, Goren EM, Mizrachi G, Bitton E, Benjamin A, Copits BA, Sasse P, Rost BR, Schmitz D, Bruchas MR, Soba P, Oren-Suissa M, Nir Y, Wiegert JS, Yizhar O
Nat Methods. 2024 May 29. doi: 10.1038/s41592-024-02285-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 198507 | pcDNA3.1-CMV-PdCO-mScarlet | Expresses optimized PdCO in frame with mScarlet under control of CMV promotor. |
| 198508 | pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor. |
| 198509 | pAAV-hSyn-SIO-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination. |
| 198510 | pAAV_hSyn-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor. |
| 198511 | pAAV_hSyn-DIO-PdCO-mScarlet-WPRE | Expresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination. |
| 198512 | pcDNA3.1-CMV-PdCO-EGFP | Expresses optimized PdCO in frame with EGFP under control of CMV promotor. |
| 198513 | pAAV_hSyn-PdCO-EGFP-WPRE | Expresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor. |
| 198514 | pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE | Expresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor. |
| 198515 | pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE | Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination. |
| 198516 | pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE | Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Cre-dependent recombination. |
| 202198 | pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE | Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor |
| 202199 | pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE | Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombination |