Universal loop assembly: open, efficient and cross-kingdom DNA fabrication.

Pollak B, Matute T, Nunez I, Cerda A, Lopez C, Vargas V, Kan A, Bielinski V, von Dassow P, Dupont CL, Federici F
Synth Biol (Oxf). 2020;5(1):ysaa001. doi: 10.1093/synbio/ysaa001. Epub 2020 Feb 5. PubMed Article

These materials are included in the Diatom uLoop Assembly Kit.

Plasmids from Article

ID	Plasmid	Purpose
202091	pCA_o-1	Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.
202092	pCA_o-2	Receiver cloning vector for odd-2 level constructs. Contains lacZ for blue/white screening.
202093	pCA_o-3	Receiver cloning vector for odd-3 level constructs. Contains lacZ for blue/white screening.
202094	pCA_o-4	Receiver cloning vector for odd-4 level constructs. Contains lacZ for blue/white screening.
202095	pL0R_lacZ	Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.
202096	pCA_e-1	Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.
202097	pCA_e-2	Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.
202098	pCA_e-3	Receiver cloning vector for even-3 level constructs. Contains lacZ for blue/white screening.
202099	pCA_e-4	Receiver cloning vector for even-4 level constructs. Contains lacZ for blue/white screening.
202100	pL0_AB-H4	Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.
202101	pL0_AB-NR	Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.
202102	pL0_AB-P49	Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.
202103	pL0_AB-OEE3	Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.
202104	pL0_AC-epyC	Level 0 plasmid containing the promoter from gene 41316 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202105	pL0_AC-BCA	Level 0 plasmid containing the promoter from gene 51305 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202106	pL0_AC-47740	Level 0 plasmid containing the promoter from gene 47740 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202107	pL0_AC-GEF	Level 0 plasmid containing the promoter from gene 41365 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202108	pL0_AC-51183	Level 0 plasmid containing the promoter from gene 51183 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202109	pL0_AC-54730	Level 0 plasmid containing the promoter from gene 54730 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202110	pL0_AC-27877	Level 0 plasmid containing the promoter from gene 27877 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202111	pL0_AC-fbaC5	Level 0 plasmid containing the promoter from gene 41423 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202112	pL0_AC-CaMK2	Level 0 plasmid containing the promoter from gene 39236 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202113	pL0_AC-act1	Level 0 plasmid containing the promoter from gene 29812 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202114	pL0_AC-sit4	Level 0 plasmid containing the promoter from gene 48707 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202115	pL0_AC-NRT	Level 0 plasmid containing the promoter from gene 54101 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202116	pL0_AC-HO	Level 0 plasmid containing the promoter from gene 5902 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202117	pL0_AC-lysA	Level 0 plasmid containing the promoter from gene 21592 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202118	pL0_AC-vapol	Level 0 plasmid containing the promoter from gene 47434 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202119	pL0_AC-fcpF	Level 0 plasmid containing the promoter from gene 51230 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202120	pL0_AC-P49	Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202121	pL0_AC-fcpB	Level 0 plasmid containing the promoter from gene 25172 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202122	pL0_AC-H4	Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202123	pL0_AC-NR	Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202124	pL0_AC-GPI1	Level 0 plasmid containing the promoter from gene 23924 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202125	pL0_AC-Fld	Level 0 plasmid containing the promoter from gene 23658 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202126	pL0_AC-dph1	Level 0 plasmid containing the promoter from gene 54330 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202127	pL0_AC-glna	Level 0 plasmid containing the promoter from gene 22357 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202128	pL0_AC-acc2	Level 0 plasmid containing the promoter from gene 55209 from Phaeodactylum tricornutum with AC overhangs used to build a level 1 construct.
202129	pL0_AC-CEN_ARS_HIS	Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.
202130	pL0_AF-spacer	Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.
202131	pL0_BC-chl_sig_pep	Level 0 plasmid containing a kozak consensus sequence and a chloroplast signal peptide sequence with BC overhangs used to build level 1 constructs.
202132	pL0_BC-pyr_comp_sig_pep	Level 0 plasmid containing a kozak consensus sequence and a pyrenoid compartment signal peptide sequence with BC overhangs used to build level 1 constructs.
202133	pL0_BC-sec_sig_pep	Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.
202134	pL0_BC-mito_sig_pep	Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.
202135	pL0_BC-nuc_sig_pep	Level 0 plasmid containing a kozak consensus sequence and a nucleus signal peptide sequence with BC overhangs used to build level 1 constructs.
202136	pL0_BC-ER_sig_pep	Level 0 plasmid containing a kozak consensus sequence and a ER signal peptide sequence with BC overhangs used to build level 1 constructs.
202137	pL0_CD-GUS	Level 0 plasmid containing a beta glucuronidase reporter gene with CD overhangs used to build a level 1 construct.
202138	pL0_CD-PT2A	Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs
202139	pL0_CD-eYFP	Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.
202140	pL0_CD-roGFP	Level 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.
202141	pL0_CD-greenlantern	Level 0 plasmid containing a green lantern fluorescent protein with CD overhangs used to build a level 1 construct.
202142	pL0_CD-eCFP	Level 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.
202143	pL0_CD-mcherry	Level 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct.
202144	pL0_CD-eYGFP-uv	Level 0 plasmid containing an eYGFP-uv fluorescent protein with CD overhangs used to build a level 1 construct.
202145	pL0_CD-mRFP1	Level 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.
202146	pL0_CD-mTurquoise2	Level 0 plasmid containing a mTurquoise2 fluorescent protein with CD overhangs used to build a level 1 construct.
202147	pL0_CD-sfGFP	Level 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.
202148	pL0_CD-Venus	Level 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct.
202149	pL0_CD-eGFP	Level 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct.
202150	pL0_CE-OriT	Level 0 plasmid containing an origin of transfer sequence from pPtPBR11 required for making plasmids mobilizable for conjugation with CE overhangs used to build level 1 constructs.
202151	pL0_DE-3xStop	Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.
202152	pL0_DE-Per_sig_pep	Level 0 plasmid containing a peroxisome signal peptide sequence with DE overhangs used to build level 1 constructs.
202153	pL0_DE-mRuby3	Level 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct.
202154	pL0_DE-RFP1	Level 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.
202155	pL0_DE_mTurquoise2	Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.
202156	pL0_DE-sfGFP	Level 0 plasmid containing a sfGFP fluorescent protein with DE overhangs used to build a level 1 construct.
202157	pL0_DE-Venus	Level 0 plasmid containing a Venus fluorescent protein with DE overhangs used to build a level 1 construct.
202158	pL0_EF-BCA	Level 0 plasmid containing a terminator sequence from gene 51305 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202159	pL0_EF-47740	Level 0 plasmid containing a terminator sequence from gene 47740 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202160	pL0_EF-GEF	Level 0 plasmid containing a terminator sequence from gene 41365 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202161	pL0_EF-51183	Level 0 plasmid containing a terminator sequence from gene 51183 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202162	pL0_EF-27877	Level 0 plasmid containing a terminator sequence from gene 27877 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202163	pL0-EF-54730	Level 0 plasmid containing a terminator sequence from gene 54730 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202164	pL0_EF-fcpA	Level 0 plasmid containing a terminator sequence from gene 18049 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202165	pL0_EF-OEE3	Level 0 plasmid containing a terminator sequence from gene 54499 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202166	pL0_EF-fbaC5	Level 0 plasmid containing a terminator sequence from gene 41423 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202167	pL0_EF-CaMK2	Level 0 plasmid containing a terminator sequence from gene 39236 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202168	pL0_EF-sit4	Level 0 plasmid containing a terminator sequence from gene 48707 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202169	pL0_EF-NRT	Level 0 plasmid containing a terminator sequence from gene 54101 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202170	pL0_EF-HO	Level 0 plasmid containing a terminator sequence from gene 5902 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202171	pL0_EF-act1_tr	Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202172	pL0_EF-act1_full	Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202173	pL0_EF-lysA	Level 0 plasmid containing a terminator sequence from gene 21592 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202174	pL0_EF-vapol	Level 0 plasmid containing a terminator sequence from gene 47434 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202175	pL0_EF-GPI1	Level 0 plasmid containing a terminator sequence from gene 23924 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202176	pL0_EF-P49	Level 0 plasmid containing a terminator sequence from gene 49202 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202177	pL0_EF-fcpB	Level 0 plasmid containing a terminator sequence from gene 25172 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202178	pL0_EF-H4	Level 0 plasmid containing a terminator sequence from gene 34971 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202179	pL0_EF-NR	Level 0 plasmid containing a terminator sequence from gene 54983 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202180	pL0_EF-PtBle	Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.
202181	pL0_EF-Fld	Level 0 plasmid containing a terminator sequence from gene 23658 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202182	pL0_EF-dph1	Level 0 plasmid containing a terminator sequence from gene 54330 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202183	pL0_EF-glna	Level 0 plasmid containing a terminator sequence from gene 22357 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202184	pL0_EF-acc2	Level 0 plasmid containing a terminator sequence from gene 41316 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.
202185	pL0_EF-epyC	Level 0 plasmid containing a terminator sequence from gene 55209 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.