Efficient and rapid fluorescent protein knock-in with universal donors in mouse embryonic stem cells.

Shi Y, Kopparapu N, Ohler L, Dickinson DJ
Development. 2023 May 15;150(10):dev201367. doi: 10.1242/dev.201367. Epub 2023 May 25. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
202078	pDD425	Frame +0 selector for NHEJ knock-in
202079	pDD426	Frame +1/-0 selector for NHEJ knock-in
202080	pDD427	Frame +2/-1 selector for NHEJ knock-in
202081	pDD428	Cas9/sgRNA plasmid targeting the C-terminus of Myh9
202082	pYS16	Parent vector for producing universal minicircle donors for mNeonGreen C-terminal knock-in
202083	pYS17	Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in
202084	pYS19	Frame -2 selector for NHEJ knock-in
202085	pYS28	Parent vector for producing universal minicircle donors for HaloTag C-terminal knock-in
202086	pYS29	Parent vector for producing universal minicircle donors for HaloTag N-terminal knock-in