In vivo human T cell engineering with enveloped delivery vehicles.
Hamilton JR, Chen E, Perez BS, Sandoval Espinoza CR, Kang MH, Trinidad M, Ngo W, Doudna JA
Nat Biotechnol. 2024 Jan 11. doi: 10.1038/s41587-023-02085-z.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 201912 | pJRH-1362 scFv entry plasmid | Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter) |
| 201913 | pJRH-1187 VSVGmut | Expresses a VSV-G variant (K47Q R354A) defective for LDL-R receptor binding (CAG promoter) |
| 201914 | pJRH-1179 U6-reci Gag-Cas9 v2 | Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion) |
| 201915 | pJRH-1180 U6-reci Gag-pol v2 | Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion) |
| 201916 | pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2 | Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA) |
| 201917 | pJRH-1346 U6-B2M sgRNA Gag-pol v2 | Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA) |
| 201918 | pJRH-1328 LV EF1a-CD20 IRES-EGFP | 2nd generation lentiviral transfer plasmid for engineering cells to express human CD20 |
| 201919 | pJRH-1363 LV EF1a-CD19 IRES-EGFP | 2nd generation lentiviral transfer plasmid for engineering cells to express human CD19 |
| 201920 | pJRH-1364 LV EF1a-CD4 IRES-EGFP | 2nd generation lentiviral transfer plasmid for engineering cells to express human CD4 |
| 201921 | pJRH-1365 LV EF1a-CD28 IRES-EGFP | 2nd generation lentiviral transfer plasmid for engineering cells to express human CD28 |