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Cell-Type-Specific Intracellular Protein Delivery with Inactivated Botulinum Neurotoxin.

Roh H, Dorner BG, Ting AY
J Am Chem Soc. 2023 May 10;145(18):10220-10226. doi: 10.1021/jacs.3c01145. Epub 2023 Apr 26. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
199714pAAV_CMV::hTfR-GFPExpresses a chimera of human transferrin receptor (hTfR) and GFP in mammalian cells.
199715pLX304_CMV::mCherry-LgBiT-FLAG-NESExpresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.
199716pLX208_CMV::mCherry-myc-VAMP2Expresses VAMP2, fused to mCherry, in mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.
199717pAAV_CMV::V5-EGFRExpresses V5-tagged EGFR in mammalian cells.

Antibodies from Article