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Protocol for analyzing intact mRNA poly(A) tail length using nanopore direct RNA sequencing.

Ogami K, Oishi Y, Hoshino SI
STAR Protoc. 2023 May 26;4(2):102340. doi: 10.1016/j.xpro.2023.102340. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
199440pGEX6P-1-eIF4E K119ABacterial expression plasmids for production of recombinant GST-eIF4E K119A

Antibodies from Article