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Addgene

A toolkit for recombinant production of seven human EGF family growth factors in active conformation.

Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, Zanchin NIT
Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
199229pET32a-hAREGBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
199230pET32a-hBTCBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
199231pET32a-hEGFBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
199232pET32a-hEPGNBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
199233pET32a-hEPRBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
199234pET32a-hHBEGFBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
199236pET32a-hTGFaBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Antibodies from Article