pET32a-hAREG
(Plasmid
#199229)
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PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 199229 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepET32a(+)
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5863
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAmphiregulin preproprotein
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Alt nameARB
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Alt nameCRDGF
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SpeciesH. sapiens (human)
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Insert Size (bp)291
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Entrez GeneAREG (a.k.a. AR, AREGB, CRDGF, SDGF)
- Promoter T7
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Tag
/ Fusion Protein
- TrxA-6xHis-S-tag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer T7 promoter
- 3′ sequencing primer T7 terminator (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET32a-hAREG was a gift from Nilson Zanchin (Addgene plasmid # 199229 ; http://n2t.net/addgene:199229 ; RRID:Addgene_199229) -
For your References section:
A toolkit for recombinant production of seven human EGF family growth factors in active conformation. Ferreira AS, Lopacinski A, Batista M, Hiraiwa PM, Guimaraes BG, Zanchin NIT. Sci Rep. 2022 Mar 23;12(1):5034. doi: 10.1038/s41598-022-09060-9. 10.1038/s41598-022-09060-9 PubMed 35322149