A scalable framework for high-throughput identification of functional origins of replication in non-model bacteria

Gilbert C, Brumwell SL, Crits-Christoph A, Kang SC, Martin-Moldes Z, Alsharif W, Esmurria A, Nguyen M-A, Lee HH, Ostrov N
bioRxiv 2023.05.19.541510 Article

These plasmids are available to request as a set: Cultivarium POSSUM Toolkit.

Plasmids from Article

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ID	Plasmid	Purpose
199109	pGL2_177	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199108	pGL2_176	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199107	pGL2_175	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199106	pGL2_174	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199105	pGL2_173	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199104	pGL2_172	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199103	pGL2_171	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199102	pGL2_170	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199101	pGL2_169	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter
199100	pGL2_168	Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter