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AP-4 mediates export of ATG9A from the trans-Golgi network to promote autophagosome formation.

Mattera R, Park SY, De Pace R, Guardia CM, Bonifacino JS
Proc Natl Acad Sci U S A. 2017 Dec 12;114(50):E10697-E10706. doi: 10.1073/pnas.1717327114. Epub 2017 Nov 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
198529pEGFPN1-ATG9AExpression of ATG9A-GFP fusion protein in mammalian cells
198530pGBT9-ATG9A-NExpression of GAL4 DNA-binding domain (BD)-ATG9A-N terminal cytosolic domain fusion protein in yeast (yeast two-hybrid assays)
198531pCI-neo-(HA)3-εExpression of HA-tagged AP-4 ε in mammalian cells

Antibodies from Article