Skip to main content
Addgene

An E3 ligase network engages GCN1 to promote the degradation of translation factors on stalled ribosomes.

Oltion K, Carelli JD, Yang T, See SK, Wang HY, Kampmann M, Taunton J
Cell. 2023 Jan 19;186(2):346-362.e17. doi: 10.1016/j.cell.2022.12.025. Epub 2023 Jan 12. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
198383pLX304 Flag-mCherry-eEF1A1_IRES-GFPeEF1A1 fluorescent reporter
198384pHR V5-RNF14_IRES-mCherryRNF14 expression
198385pHR V5-RNF14 C417A_IRES-mCherryRNF14 C417A expression
198386pHR V5-RNF25_IRES-mCherryRNF25 expression
198387pHR V5-RNF25 dRING_IRES-mCherryRNF25 (?RING) expression
198388pLenti6.3 GCN1-3xFlag_IRES-iRFPGCN1 expression
198389pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFPGCN1 R2312A expresson
198390pHR RPS27A-3xFlag_IRES-GFPRPS27A (ubiquitin/ribosomal protein eS31 fusion) expression
198391pHR RPS27A K113R-3xFlag_IRES-GFPRPS27A K113R (ubiquitin/ribosomal protein eS31 fusion) expression
198392pHR RPS27A K107/113R-3xFlag_IRES-GFPRPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression
198393pLX304 3xFlag-eRF1_IRES-GFPeRF1 expression
198394pLX304 3xFlag-eRF1 G183/184A_IRES-GFPeRF1 (AAQ mutant) expression

Antibodies from Article