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Spatial organization of the 3D genome encodes gene co-expression programs in single cells

Dong P, Zhang S, Xie L, Wang L, Lemire AL, Lander AD, Chang HY, Liu ZJ
bioRxiv 2022.10.26.513917 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197865pminiT-MED6-mEmerald donorDonor plasmid used for building MED6-mEmerald knock-in mESCs
197866pminiT-MED6-Halo donorDonor plasmid used for building MED6-Halo knock-in mESCs
197867pminiT-mEmerald-MED1 donorDonor plasmid used for building mEmerald-MED1 knock-in mESCs
197868pminiT-Halo-MED1 donorDonor plasmid used for building Halo-MED1 knock-in mESCs
197869pTij_sg_mmu_Med6_C_terminalCRISPR/Cas9/sgRNA plasmid for cutting Med6 C terminal and building MED6-mEmerald/Halo knock-in mESCs
197870pTij_sg_mmu_Med1_N_terminalCRISPR/Cas9/sgRNA plasmid for cutting Med1 N terminal and building mEmerald/Halo-MED1 knock-in mESCs
197907piggybac-EF1-H2AZ-halosingle-particle tracking for H2AZ
197908piggybac-EF1-halo-mTBPsingle-particle tracking for mTBP
197909piggybac-EF1-halo-Oct4single-particle tracking for OCT4

Antibodies from Article