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Tuning Methylation-Dependent Silencing Dynamics by Synthetic Modulation of CpG Density.

Ma Y, Budde MW, Zhu J, Elowitz MB
ACS Synth Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
197677vYM004_pNeo_phiC31_nTRE_pEF1s(orig)_citrine"pEF1s(orig)" reporter plasmid
197678vYM010_phiC31-nTRE-pEF1s(high)-H2b-Citrine"pEF1s(high)" reporter plasmid
197679vYM011_phiC31-nTRE-pEF1s(low)-H2b-Citrine"pEF1s(low)" reporter plasmid
197680vYM012_phiC31-nTRE-Ins(high)-pEF1s(high)-H2b-Citine"Insert(high)-pEF1s(high)" reporter plasmid
197681vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine"Insert(high)-pEF1s(orig)" reporter plasmid
197682vYM014_phiC31-nTRE-Ins(low)-pEF1s(low)-H2b-Citrine"Insert(low)-pEF1s(low)" reporter plasmid
197683vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine"Insert(low)-pEF1s(orig)" reporter plasmid
197684vYM141_phiC31_nTRE-pEF1s(orig)(CC793)-H2B-citrine"pEF1s(orig)" reporter plasmid, with CpG793 mutated to CC
197685vYM142_phiC31-nTRE-pEF1s(high)(CC793)-H2B-citrine"pEF1s(high)" reporter plasmid, with CpG793 mutated to CC
197686vYM143_phiC31-nTRE-pEF1s(low)(CG793)-H2b-Citrine"pEF1s(low)" reporter plasmid, with CpG793 mutated to CG
197687vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCDplasmid for expressing the DNMT3bCD
202596vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCIplasmid for expressing the DNMT3bCI (catalytic inactive)

Antibodies from Article