vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI
(Plasmid
#202596)
-
Purposeplasmid for expressing the DNMT3bCI (catalytic inactive)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202596 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonePiggyBac
- Backbone size w/o insert (bp) 6300
- Total vector size (bp) 10800
-
Vector typeMammalian Expression, Synthetic Biology
-
Selectable markersBlasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature30°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameH2B-mCherry-T2A-rTetR-DNMT3bCI
-
SpeciesM. musculus (mouse), Synthetic
-
Insert Size (bp)4500
-
MutationP656V, C657D
-
GenBank IDNM_001003961
-
Entrez GeneDnmt3b (a.k.a. MmuIIIB)
-
Tag
/ Fusion Protein
- rTetR (N terminal on insert)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.05.30.542205 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
vYM245_PB-Blast-pEF1a-H2B-mCh-D3bCI was a gift from Michael Elowitz (Addgene plasmid # 202596 ; http://n2t.net/addgene:202596 ; RRID:Addgene_202596) -
For your References section:
Tuning Methylation-Dependent Silencing Dynamics by Synthetic Modulation of CpG Density. Ma Y, Budde MW, Zhu J, Elowitz MB. ACS Synth Biol. 2023 Aug 12. doi: 10.1021/acssynbio.3c00078. 10.1021/acssynbio.3c00078 PubMed 37572041
Map uploaded by the depositor.
