Growth cone advance requires EB1 as revealed by genomic replacement with a light-sensitive variant.
Dema A, Charafeddine R, Rahgozar S, van Haren J, Wittmann T
Elife. 2023 Jan 30;12:e84143. doi: 10.7554/eLife.84143.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 197419 | pEB1N-mScarlet-LZ-LOV2 | Mammalian expression of the mScarlet-tagged N-terminal half of photo-inactivated π-EB1 |
| 197420 | pSpCas9(BB)-2A-mCherry | Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA |
| 197421 | pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1 | Knock out of EB3 in human cells by CRISPR/Cas9 |
| 197422 | pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#2 | Knock out of EB3 in human cells by CRISPR/Cas9 |
| 197423 | pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3 | Knock out of EB3 in human cells by CRISPR/Cas9 |
| 197424 | pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1 | π-element knock-in into exon 5 of MAPRE1 |
| 197425 | pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2 | π-element knock-in into exon 5 of MAPRE1 |
| 197426 | pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#3 | π-element knock-in into exon 5 of MAPRE1 |
| 197427 | pUC19-π-EB1_IRES-mCherry | Homology-directed repair template targeting IRES-based π-element to MAPRE1 exon 5 |
| 197429 | pUC19-π-EB1_EF1α-mCherry | Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5 |
| 197430 | pUC19-π-EB1_EF1α-EGFP | Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5 |