The Rab5 guanine nucleotide exchange factor Rabex-5 binds ubiquitin (Ub) and functions as a Ub ligase through an atypical Ub-interacting motif and a zinc finger domain.
Mattera R, Tsai YC, Weissman AM, Bonifacino JS
J Biol Chem. 2006 Mar 10;281(10):6874-83. doi: 10.1074/jbc.M509939200. Epub 2006 Jan 5.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196937 | pCI-neo-Myc-Rabex-5 | Expresses Myc-tagged Rabex-5 in mammalian cells |
| 196991 | pCI-neo-(HA)3-ubiquitin | Expresses HA-tagged ubiquitin in mammalian cells |
| 196999 | pGBT9-ubiquitin | Expression of GAL4 DNA-binding domain (BD)-ubiquitin fusion protein in yeast (yeast two-hybrid assays) |
| 197043 | pGAD424-Rabex-5 | Expression of GAL4 transcriptional activation domain (AD)-Rabex-5 fusion protein in yeast (yeast two-hybrid assays) |