Centrosomal microtubule nucleation regulates radial migration of projection neurons independently of polarization in the developing brain.
Vinopal S, Dupraz S, Alfadil E, Pietralla T, Bendre S, Stiess M, Falk S, Camargo Ortega G, Maghelli N, Tolic IM, Smejkal J, Gotz M, Bradke F
Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196848 | pBactin-EB3-mNeonGreen | labelling of microtubule plus ends |
| 196849 | pBactin-AcGFP-CI | Expression of green fluorescent reporter AcGFP driven by the strong Beta-actin promoter |
| 196850 | pBactin-mNeonGreen-CI | Expression of green fluorescent reporter mNeonGreen driven by the strong Beta-actin promoter |
| 196851 | pBactin-td-mCherry-CI | Expression of a tandem (td) red fluorescent reporter mCherry driven by the strong Beta-actin promoter |
| 196852 | pBactin-mOrange2-CI | Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter |
| 196853 | pBactin-AcGFP-Cdk5rap2-CTD | Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to AcGFP. Used to displace endogenous Cdk5rap2 from centrosomes. |
| 196854 | pBactin-td-mCherry-Cdk5rap2-CTD | Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes. |
| 196855 | pBactin-mOrange2-Cdk5rap2-CTD | Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes. |
| 196856 | pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD | Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes |
| 196857 | pBactin-EGFP-NEDD1-gTBD | Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome. |
| 196858 | pBactin-mOrange2-NEDD1-gTBD | Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome. |
| 196859 | pBactin-td-mCherry-Cdk5rap2 | Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry. |
| 196860 | pBactin-Nedd1-mOrange2 | Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 |
| 196861 | pBactin-Nedd1-mOrange2-rCM1 | Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC. |
| 196862 | pBactin-Nedd1-mOrange2-F75A | Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC |
| 196863 | pBactin-Nedd1-mOrange2-hCM1 | Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC |
| 196864 | pBactin-Nedd1-mNeonGreen | Expression of neural precursor cell expressed developmentally down-regulated 1 (Need1) fused to mNeonGreen |
| 196865 | pBactin-Akna-mOrange2 | Expression of the centrosomal protein Akna fused to mOrange2 |
| 196866 | pBactin-Akna-mEGFP | Expression of the centrosomal protein Akna fused to enhanced (E)-GFP |
| 196867 | pCR-XL-rAkap9 | Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs |
| 196868 | pBactin-PACT-mOrange2-hCM1 | Expression of Pericentrin-AKAP450 centrosomal targeting (PACT) domain fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). PACT targets CM1 to the centrosome |
| 196869 | pBactin-Tubg1-mNeonGreen | Expression of the gamma-tubulin fused to mNeonGreen |
| 196870 | pBactin-Tubg1-mEGFP | Expression of the gamma-tubulin fused to enhanced (E)-GFP |
| 196871 | pBactin-AcGFP-128-425-Akap9 | Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to AcGFP. Used to displace endogenous Akap9 from Golgi |
| 196872 | pCAG-mScarlet-Giantin | Expresion of Giantin-fused to mScarlet. Used as Golgi marker |
| 196873 | pCAG-Halo-Tag-128-425-Akap9 | Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi |
| 196874 | pCAG-rox-inv[Talpha1-iCre-pA]-rox-LynGFP | Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-Dre-pA plasmids |
| 196875 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-Lyn-EGFP | Neuron-specific expression of LynGFP reporter |
| 196876 | pCAG-lox-inv[Talpha1-Dre-pA]-lox-Lyn-EGFP | Neuron-specific expression of LynGFP reporter. Used in combination with Talpha1-iCre-pA plasmids |
| 196877 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-C1 | Neuron-specific expression of mOrange2 reporter |
| 196878 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD | Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome. |
| 196879 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1 | Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC. |
| 196880 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-Akna-mOrange2 | Neuron-specific expression of the centrosomal protein Akna fused to mOrange2 |
| 196881 | pCAG-lox-inv[Talpha1-Dre-pA]-lox-mOrange2-Cdk5rap2-CTD | Neuron-specific expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to mOrange2. Used to displace endogenous Cdk5rap2 from centrosomes. |
| 196882 | pCAG-DIO-basic | Used for preparation of double-floxed inverse ORF vectors |
| 196883 | pCAG-DIO-inv[roxSTOProx-ZsGreen-bGH] | Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporter |
| 196884 | pCAG-DIO-inv[roxSTOProx-Lyn-GFP-bGH] | Dual Cre/Dre-dependent expression of LynGFP. Used as Cre-Dre cotrasfection reporter |
| 196885 | pCAG-DIO-inv[roxSTOProx-EB3 mNeonGreen-bGH] | Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotrasfection reporter |
| 196886 | pBactin-DIO-inv[roxSTOProx-ZsGreen-bGH] | Dual Cre/Dre-dependent expression of ZsGreen. Used as Cre-Dre cotrasfection reporter |
| 196887 | pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH] | Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotrasfection reporter |
| 196888 | pCAG-lox-roxSTOProx-tRFP-pA-lox | Dre-activated Cre-inactivated expression of turbo (t)RFP reporter. |
| 196889 | pCAG-lox-roxSTOProx-lyn-mScarlet-pA-lox | Dre-activated Cre-inactivated expression of lyn-mScarlet reporter. |
| 196890 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1 | Neuron-specific expression of human (h)Centrin fused to mNeonGreen |
| 196891 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-hCentrin1 | Neuron-specific expression of human (h)Centrin fused to 2xHA-tags |
| 196892 | pCAG-rox-inv[Talpha1-iCre-pA]-rox-mNeonGreen-hCentrin1 | Neuron-specific expression of human (h)Centrin fused to mNeonGreen. Used in combination with Talpha1-Dre-pA plasmids |
| 196893 | pCAG-lox-inv[Talpha1-HA-Dre]-lox-Akna-mOrange2 | Neuron-specific expression of Akna fused to mOrange2. Used in combination with Talpha1-iCre-pA plasmids |
| 196894 | pCAG-lox-inv[Talpha1-HA-Dre]-lox-mOrange2-NEDD1-gTBD | Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange aimed to displace endogenous gamma-TuRC from the centrosome. Used in combination with Talpha1-iCre-pA plasmids |
| 196895 | pCAG-lox-inv[Talpha1-Dre-PA]-lox-2xHA-CAMSAP3 | Neuron-specific expression of Calmodulin Regulated Spectrin Associated Protein Family Member 3 (CAMSAP3) fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids |
| 196896 | pCAG-lox-inv[Talpha1-Dre-PA]-lox-N-Cadherin-2xHA | Neuron-specific expression of N-Cadherin fused to 2xHA-tags. Used in combination with Talpha1-iCre-pA plasmids |
| 196897 | pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9 | Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags. |
| 196898 | pBactin-Halo-Tag | Expression of Halo-Tag. Use as control for Halo-tag fusions |