pCR-XL-rAkap9
(Plasmid #196867)

• Purpose: Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs
• Depositing Lab: Frank Bradke
• Publication: Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16. doi: 10.1016/j.neuron.2023.01.020. Epub 2023 Feb 15.

Backbone

• Vector backbone: pCR-XL-TOPO
• Backbone size w/o insert (bp): 3519
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 30 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Growth instructions: Grow 2-3 days at room temperature. See comments section for more details.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Akap9
• Species: R. norvegicus (rat)
• Insert Size (bp): 11505
• Entrez Gene: Akap9 (a.k.a. CG-NAP, Gisp, Yotiao)
• Promoter: Lac promoter and T7 promoter

Cloning Information

• Cloning method: TOPO Cloning

Resource Information

• Supplemental Documents:
  • pCR-XL-rAkap9.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For successful transformation of Plasmid 196867: pCR-XL-rAkap9, it is recommended to use electroporation and to grow transformed bacteria at no more than 28 °C for two days and with 30 μg/ml kanamycin on LB plates for full length plasmid production