Combining natural sequence variation with high throughput mutational data to reveal protein interaction sites.
Melamed D, Young DL, Miller CR, Fields S
PLoS Genet. 2015 Feb 11;11(2):e1004918. doi: 10.1371/journal.pgen.1004918. eCollection 2015 Feb.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196833 | pOAD eIF4G1 (1-341) | Expesses S.cerevisiae eIF4G1 amino acids 1-341 as Gal4 activation domain fusion. |
| 196834 | pOBD2 Pab1 WT (123-204) | Expresses Pab1 RRM2 fragment fused to GAL4 DNA binding domain |
| 196835 | pOBD2 Pab1 E181R (123-204) | Expresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domain |