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Temporal segregation of biosynthetic processes is responsible for metabolic oscillations during the budding yeast cell cycle.

Takhaveev V, Ozsezen S, Smith EN, Zylstra A, Chaillet ML, Chen H, Papagiannakis A, Milias-Argeitis A, Heinemann M
Nat Metab. 2023 Feb;5(2):294-313. doi: 10.1038/s42255-023-00741-x. Epub 2023 Feb 27. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
196612pROS_phleo-Tps1/Gsy2Encoding guide RNAs for the knock out of TPS1 and GSY2 genes
196613pROS13-Tps2/Gsy1Encoding guide RNAs for the knock out of TPS2 and GSY1 genes
196614GA46Plasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeast
196615pUGP1.1Plasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeast
196616pB_tetO7_sfGFPPlasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Antibodies from Article