T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation.
Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P, Tornisiello R, Parsi KM, Huey J, Mielke T, Meierhofer D, Maehr R, Hnisz D, Michor F, Rinn JL, Meissner A
Elife. 2023 Jan 31;12:e83077. doi: 10.7554/eLife.83077.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195495 | 2X_pX458_pSpCas9(BB)-2A-GFP_eSOX17.2_KO | Cas9-2A-GFP expression vector bearing two sgRNAs targeting the core endoderm distal enhancer of human SOX17 |
| 195496 | pGL4.15_pSOX17 | Firefly luciferase promoter expression vector driven only by the promoter element of human SOX17 |
| 195497 | pGL4.15_pT-REX17 | Firefly luciferase promoter expression vector driven only by the promoter element of human lncRNA T-REX17 |
| 195498 | pGL4.27_eSOX17 | Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the extended endoderm distal enhancer of human SOX17 |
| 195499 | pGL4.27_eSOX17.2 | Firefly luciferase enhancer expression vector driven by a minimal CMV-promoter and the core endoderm distal enhancer of human SOX17 |
| 195500 | pU6-sgCtrl_EF1a-Puro-T2A-BFP | Lentiviral BFP expression vector bearing a sgRNA targeting a randomized human TSS sequence |
| 195501 | pU6-sgT-REX17_EF1a-Puro-T2A-BFP | Lentiviral BFP expression vector bearing a sgRNA targeting the promoter of human T-REX17 |
| 195502 | pX335_U6-Chimeric_BB-CBh-hSpCas9n(D10A)_SOX17_Ex2_KI | Cas9 nickase expression vector bearing a sgRNA targeting Exon 2 of human SOX17 |
| 195503 | pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP | SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus |
| 195504 | pX459_V2.0_pSpCas9(BB)-2A-Puro_T-REX17_Ex1_KI | Cas9-2A-Puro expression vector bearing a sgRNA targeting Exon 1 of human lncRNA T-REX17 |
| 195505 | pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP | T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17 |