IntS6 and the Integrator phosphatase module tune the efficiency of select premature transcription termination events.
Fujiwara R, Zhai SN, Liang D, Shah AP, Tracey M, Ma XK, Fields CJ, Mendoza-Figueroa MS, Meline MC, Tatomer DC, Yang L, Wilusz JE
Mol Cell. 2023 Nov 14:S1097-2765(23)00902-4. doi: 10.1016/j.molcel.2023.10.035.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 195062 | Hy_pRnrS eGFP SV40 | Expresses eGFP mRNA from the Drosophila RnrS promoter |
| 195063 | Hy_pAna eGFP SV40 | Expresses eGFP mRNA from the Drosophila Ana promoter |
| 195064 | Hy_U5:34A eGFP SV40 | Expresses eGFP when there is readthrough transcription from the Drosophila U5:34A gene |
| 195065 | pUb FLAG-Mts | Expresses FLAG-tagged Drosophila mts protein |
| 195066 | pUb FLAG-Pp2A-29B | Expresses FLAG-tagged Drosophila Pp2A-29B protein |
| 195067 | pUb FLAG-cka | Expresses FLAG-tagged Drosophila cka protein |
| 195068 | pUb FLAG-tws | Expresses FLAG-tagged Drosophila tws protein |
| 195069 | pUb FLAG-wdb | Expresses FLAG-tagged Drosophila wdb protein |
| 195070 | pUb FLAG-wrd | Expresses FLAG-tagged Drosophila wrd protein |
| 195071 | pUb FLAG-IntS6 AA 1-400 | Expresses FLAG-tagged Drosophila IntS6 amino acids 1-400 |
| 195072 | pUb FLAG-IntS6 AA 1-600 | Expresses FLAG-tagged Drosophila IntS6 amino acids 1-600 |
| 195073 | pUb FLAG-IntS6 AA 101-1284 | Expresses FLAG-tagged Drosophila IntS6 amino acids 101-1284 |
| 195074 | pUb FLAG-IntS6 AA 1197-1284 | Expresses FLAG-tagged Drosophila IntS6 amino acids 1197-1284 |
| 195075 | pUb FLAG-IntS6 AA 101-1200 | Expresses FLAG-tagged Drosophila IntS6 amino acids 101-1200 |
| 195076 | pMtnA FLAG-IntS6 puro | Expresses FLAG-tagged Drosophila IntS6 from inducible MtnA promoter |
| 195077 | pMtnA FLAG-IntS12 puro | Expresses FLAG-tagged Drosophila IntS12 from inducible MtnA promoter |
| 196904 | pRSFDuet-1 IntS6 AA 1035-1284 | Expresses His-tagged Drosophila IntS6 amino acids 1035-1284 |
| 196905 | pRSFDuet-1 IntS8 AA 1-308 | Expresses His-tagged Drosophila IntS8 amino acids 1-308 |
| 198408 | pUb FLAG-Human IntS6 | Expresses FLAG-tagged human IntS6 |
| 198409 | pUb FLAG-Human IntS6L | Expresses FLAG-tagged human IntS6L |
| 198410 | pUb FLAG-Zebrafish IntS6 | Expresses FLAG-tagged zebrafish IntS6 |
| 198411 | pUb FLAG-Zebrafish IntS6L | Expresses FLAG-tagged zebrafish IntS6L |
| 199329 | pRSFDuet-1 IntS11 AA 300-597 | Expresses His-tagged Drosophila IntS11 amino acids 300-597 |
| 208402 | pUb FLAG-Mts H59Q | Expresses FLAG-tagged Drosophila mts protein with H59Q mutation |
| 208403 | pUb FLAG-Mts D85N | Expresses FLAG-tagged Drosophila mts protein with D85N mutation |
| 208404 | pUb FLAG-Mts H241A | Expresses FLAG-tagged Drosophila mts protein with H241A mutation |
| 208405 | pMtnA FLAG-IntS7 puro | Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter |
| 208406 | pMtnA FLAG-IntS13 puro | Expresses FLAG-tagged Drosophila IntS13 from inducible MtnA promoter |
| 208407 | pMtnA FLAG-IntS14 puro | Expresses FLAG-tagged Drosophila IntS14 from inducible MtnA promoter |
| 210521 | pGEX-6P-1 IntS12 | Expresses GST-tagged Drosophila IntS12 |