Myosin-X recruits lamellipodin to filopodia tips.
Popovic A, Miihkinen M, Ghimire S, Saup R, Gronloh MLB, Ball NJ, Goult BT, Ivaska J, Jacquemet G
J Cell Sci. 2023 Mar 1;136(5):jcs260574. doi: 10.1242/jcs.260574. Epub 2023 Mar 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 194858 | GFP-MYO10-BioID | Express MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells. |
| 194859 | GFP-RAPH1ΔTBS | Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells. |
| 194860 | eGFP-RAPH1-F1 | Express the RAPH1 fragment (1-535) tagged to eGFP in mammalian cells. |
| 194861 | eGFP-RAPH1-F2 | Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells. |
| 194862 | eGFP-RAPH1-F3 | Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells. |
| 194863 | eGFP-RAPH1-F4 | Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells. |
| 194864 | GST-RAPH1-F2 | Express the RAPH1 fragment (535-868) tagged to GST in bacteria. |
| 194865 | GFP-RAPH1Δ536-587 | Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore. |