The Mettl3 epitranscriptomic writer amplifies p53 stress responses.
Raj N, Wang M, Seoane JA, Zhao RL, Kaiser AM, Moonie NA, Demeter J, Boutelle AM, Kerr CH, Mulligan AS, Moffatt C, Zeng SX, Lu H, Barna M, Curtis C, Chang HY, Jackson PK, Attardi LD
Mol Cell. 2022 Jul 7;82(13):2370-2384.e10. doi: 10.1016/j.molcel.2022.04.010. Epub 2022 May 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 196262 | pCS2-HA-Mettl3 | In vitro transcription/translation of HA-tagged wild-type Mettl3 |
| 196263 | Lenti-U6-sgMettl3 | Expresses a Mettl3-targeting sgRNA driven by the U6 promoter and Cre-recombinase driven by the PGK promoter |
| 196267 | pcDNA3.1-3XHA-p53 | Mammalian expression of 3xHA tagged wild-type p53 |
| 196268 | pcDNA3.1-3XHA-p53 QM | Mammalian expression of 3xHA tagged p53 L25Q,Q26S,F53Q,F54S |
| 196269 | pcDNA3.1-3XHA-p53 Δ1-42 | Mammalian expression of 3xHA tagged p53 Δ1-42 (ΔTAD1) |