Directed differentiation of human iPSCs to functional ovarian granulosa-like cells via transcription factor overexpression.
Pierson Smela MD, Kramme CC, Fortuna PRJ, Adams JL, Su R, Dong E, Kobayashi M, Brixi G, Kavirayuni VS, Tysinger E, Kohman RE, Shioda T, Chatterjee P, Church GM
Elife. 2023 Feb 21;12:e83291. doi: 10.7554/eLife.83291.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192892 | pHDR-FOXL2-T2A-tdTomato-PuroTK | HDR donor for inserting FOXL2-T2A-tdTomato reporter |
| 192893 | pX330-FOXL2-cterm | Expresses Cas9 and sgRNA targeting the C-terminus region of FOXL2 |
| 192894 | pPB-cT3G-cERP2-TCF21 | Barcoded piggybac transposon vector with Dox-inducible expression of TCF21 |
| 192895 | pPB-cT3G-cERP2-JUN | Barcoded piggybac transposon vector with Dox-inducible expression of JUN |
| 192896 | pPB-cT3G-cERP2-GATA4 | Barcoded piggybac transposon vector with Dox-inducible expression of GATA4 |
| 192897 | pPB-cT3G-cERP2-CEBPD | Barcoded piggybac transposon vector with Dox-inducible expression of CEBPD |
| 192898 | pPB-cT3G-cERP2-EMX2 | Barcoded piggybac transposon vector with Dox-inducible expression of EMX2 |
| 192899 | pPB-cT3G-cERP2-MYC | Barcoded piggybac transposon vector with Dox-inducible expression of MYC |
| 192900 | pPB-cT3G-cERP2-NR2F2 | Barcoded piggybac transposon vector with Dox-inducible expression of NR2F2 |
| 192901 | pPB-cT3G-cERP2-FOSB | Barcoded piggybac transposon vector with Dox-inducible expression of FOSB |
| 192903 | pPB-cT3G-cERP2-ZBTB16 | Barcoded piggybac transposon vector with Dox-inducible expression of ZBTB16 |
| 192904 | pPB-cT3G-cERP2-YBX1 | Barcoded piggybac transposon vector with Dox-inducible expression of YBX1 |
| 192905 | pPB-cT3G-cERP2-LHX1 | Barcoded piggybac transposon vector with Dox-inducible expression of LHX1 |
| 192906 | pPB-cT3G-cERP2-WT1 | Barcoded piggybac transposon vector with Dox-inducible expression of WT1 |
| 192907 | pPB-cT3G-cERP2-JUNB | Barcoded piggybac transposon vector with Dox-inducible expression of JUNB |
| 192908 | pPB-cT3G-cERP2-KLF4 | Barcoded piggybac transposon vector with Dox-inducible expression of KLF4 |
| 192909 | pPB-cT3G-cERP2-KLF6 | Barcoded piggybac transposon vector with Dox-inducible expression of KLF6 |
| 192910 | pPB-cT3G-cERP2-HOXC9 | Barcoded piggybac transposon vector with Dox-inducible expression of HOXC9 |
| 192911 | pPB-cT3G-cERP2-ATF4 | Barcoded piggybac transposon vector with Dox-inducible expression of ATF4 |
| 192912 | pPB-cT3G-cERP2-PPARG | Barcoded piggybac transposon vector with Dox-inducible expression of PPARG |
| 192913 | pPB-cT3G-cERP2-EGR1 | Barcoded piggybac transposon vector with Dox-inducible expression of EGR1 |
| 192914 | pPB-cT3G-cERP2-FOXL2 | Barcoded piggybac transposon vector with Dox-inducible expression of FOXL2 |
| 192915 | pPB-cT3G-cERP2-NR5A1 | Barcoded piggybac transposon vector with Dox-inducible expression of NR5A1 |
| 192916 | pPB-cT3G-cERP2-TSC22D3 | Barcoded piggybac transposon vector with Dox-inducible expression of TSC22D3 |
| 192917 | pPB-cT3G-cERP2-MAFF | Barcoded piggybac transposon vector with Dox-inducible expression of MAFF |
| 192918 | pPB-cT3G-cERP2-ELK1 | Barcoded piggybac transposon vector with Dox-inducible expression of ELK1 |
| 192919 | pPB-cT3G-cERP2-NR1H2 | Barcoded piggybac transposon vector with Dox-inducible expression of NR1H2 |
| 192920 | pPB-cT3G-cERP2-TOX2 | Barcoded piggybac transposon vector with Dox-inducible expression of TOX2 |
| 192921 | pPB-cT3G-cERP2-LHX9 | Barcoded piggybac transposon vector with Dox-inducible expression of LHX9 |
| 192922 | pPB-cT3G-cERP2-ZFPM2 | Barcoded piggybac transposon vector with Dox-inducible expression of ZFPM2 |
| 192923 | pPB-cT3G-cERP2-HOPX | Barcoded piggybac transposon vector with Dox-inducible expression of HOPX |
| 192924 | pPB-cT3G-cERP2-FOS | Barcoded piggybac transposon vector with Dox-inducible expression of FOS |
| 192925 | pPB-cT3G-cERP2-NR4A1 | Barcoded piggybac transposon vector with Dox-inducible expression of NR4A1 |
| 192926 | pPB-cT3G-cERP2-RUNX2 | Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2 |
| 192927 | pPB-cT3G-cERP2-TAF4B | Barcoded piggybac transposon vector with Dox-inducible expression of TAF4B |
| 192928 | pPB-cT3G-cERP2-RUNX1 | Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1 |